Background: Diabetes mellitus is a class of metabolic disorder which results high level of sugar due to inadequacy in insulin secretion. High Sugar level in diabetes is linked with the impairment and dysfunction of eyes, kidneys, blood vessels, nerves and heart. In current research there is extended interest in herbal medicines because of the side effects noticed with oral hypoglycemic for therapy of hyperglycemia. Herbal medicines could play a dominant role in the control of diabetes mellitus. Methods: Methanolic extracts of seeds of Psoralea corylifolia L & Psoralea esculenta L has been screened for both in-vitro and in-vivo antihyperglycemic role using streptozotocin induced diabetic rats. The methanolic extract of both plants were evaluated for pancreatic tissue studies. Results: According to the outcomes, a stunning blood glucose level decrement was observed in the diabetic groups treated by methanolic extracts of Psoralea corylifolia L. seeds. Conclusion: In this study, the methanolic extract of Psoralea corylifolia L. seeds used to evaluate antihyperglycemic potential showed appreciably notable and astonishing results, when compared with the Glibenclamide. Methanolic extract of Psoralea corylifolia L. seeds have potential to regulate hyperglycemia in the In-vivo model. Hence Psoralea corylifolia L. methanolic seeds extract may be selected for its antihyperglycemic activity.