Zinc ion (Zn) not only plays an important function in the structural, catalytic, transcription, and regulatory of proteins, but is also an essential ionic signal to regulate brain neurotransmitters pass process. In this work, we designed and synthesized an intramolecular charge transfer-based ratiometric two-photon fluorescence probe, P-Zn, for imaging and biosensing of Zn in live cell, hippocampal tissue, and zebrafish. The developed probe demonstrated high two-photon absorption cross section (δ) of 516 ± 77 GM, which increased to 958 ± 144 GM after the probe was coordinated with Zn. Furthermore, this P-Zn probe quickly recognized Zn with high selectivity, over other metal ions, amino acids, and reactive oxygen species. More interestingly, the initial emission peak of the present probe at 465 nm decreased with a new peak increased at 550 nm, leading to the ratiometric determination of Zn with high accuracy. Finally, this two-photon fluorescence probe with high temporal resolution and remarkable analytical performance, as well as low-cytotoxicity, was successfully applied in imaging of live cells, hippocampal tissues, and zebrafishes. The present P-Zn probe combined with FLIM provided accurate mapping of Zn distribution at single-cell level. More interestingly, the two-photon spectroscopic results demonstrated that the level of Zn in hippocampal tissue of mouse with AD was higher than that in normal mouse brain.
Phototherapy
is an effective strategy to control Candida
albicans (C. albicans) infection without
raising the concern of drug resistance. Despite its effectiveness,
a higher dose of phototherapeutic power is required for C.
albicans elimination compared to bacteria that have to be
used, which is readily accompanied by off-target heat and toxic singlet
oxygen to damage normal cells, thus limiting its usefulness for antifungal
applications. Here to overcome this, we develop a “three-in-one”
biomimetic nanoplatform consisting of an oxygen-dissolved perfluorocarbon
camouflaged by a photosensitizer-loaded vaginal epithelial cell membrane.
With a cell membrane coating, the nanoplatform is capable of specifically
binding with C. albicans at the superficial or deep
vaginal epithelium, thereby centering the phototherapeutic agents
on C. albicans. Meanwhile, the cell membrane coating
endows the nanoplatform to competitively protect healthy cells from
candidalysin-medicated cytotoxicity. Upon candidalysin sequestration,
pore-forming on the surface of the nanoplatform accelerates release
of the preloaded photosensitizer and oxygen, resulting in enhanced
phototherapeutic power for improved anti-C. albicans efficacy under near-infrared irradiation. In an intravaginal C. albicans-infected murine model, treatment with the nanoplatform
leads to a significantly decreased C. albicans burden,
particularly when leveraging candidalysin for further elevated phototherapy
and C. albicans inhibition. Also, the same trends
hold true when using the nanoplatform to treat the clinical C. albicans isolates. Overall, this biomimetic nanoplatform
can target and bind with C. albicans and simultaneously
neutralize the candidalysin and then transform such toxins that are
always considered a positive part in driving C. albicans infection with the power of enhancing phototherapy for improved
anti-C. albicans efficacy.
Aim: A sensitive and selective ultra performance LC–MS/MS (UPLC–MS/MS) method was developed to investigate the pharmacokinetics of rosavin as a potential adaptogenic drug isolated from Rhodiola rosa L. in rat plasma with salidroside as an internal standard. Methodology: Chromatographic separation was performed on a UPLC HSS T3 column (1.8 μm, 100 mm × 2.1 mm) with gradient elution. Multiple reaction monitoring was employed for MS analysis. Rosavin and salidroside were determined with multiple reaction monitoring–ion transitions m/z 427.2 → 293.1 and m/z 299.1 → 119.1, respectively. Conclusion: The validated UPLC–MS/MS method showed a satisfied linear range in 5–5000 ng/ml-1 and was successfully applied for the pharmacokinetic study of rosavin in the rat after intravenous and gavage administration.
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