In this study, b-cyclodextrin (b-CD) was covalently grafted on hydroxyapatite (HA) using a coupling agent to improve the drug loading capacity and prolong the drug release. The binding of b-CD on the HA surface was confirmed by Fourier transformation infrared spectroscopy, thermal gravimetric analysis, and X-ray powder diffraction. The adsorption capacity of ofloxacin on b-CD-grafted hydroxyapatite (b-CD-g-HA) composite was found to be 30 mg g À1 at 37 C and 24 h. The adsorption process is spontaneous, given the negative values of free energy change. Compared with the release of ofloxacin loaded on HA, the release of ofloxacin loaded on b-CD-g-HA was slowed down 28% and 21% in pH 2.0 and pH 7.4 buffer media at 2 h, respectively. Biocompatibility of b-CD-g-HA was assessed by MTT assay, and the result showed that it had no cytotoxicity.
A highly selective imprinted sorbent was prepared by a surface molecular imprinting technique, and used for solid phase extraction-high performance liquid chromatography (SPE-HPLC) to determine trace norfloxacin (NOF) in complicated samples. The molecularly imprinted polymer (MIP) was obtained by using NOF as the template, b-cyclodextrin-methyl methacrylate (b-CD-MMA) and acrylamide (AM) as functional monomers, ethylene glycol dimethacrylate (EDMA) as the cross-linking agent and toluene as the porogen. Various parameters affecting the extraction efficiency of the polymer have been evaluated to optimize the selective preconcentration of the NOF from fish samples. The NOF from fish samples, selectively extracted by MIP, was detected by RP-HPLC. The MISPE-HPLC method showed high selectivity and good recoveries (>85.7%). It was demonstrated that this MISPE-HPLC method could be applied for direct preconcentration and determination of quinolones in real samples.
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