Over the 42 month period from January 2003 to June 2006, a total of 2,952 canine biopsy specimens were received from the Veterinary Medical Teaching Hospital of Seoul National University and from veterinary practitioners across the nation. Out of these, 748 (25.34%) cases were diagnosed as canine cutaneous tumors in the Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Korea. Thirty-eight different types of cutaneous tumors were identified and categorized into epithelial and melanocytic tumors (56.95%), mesenchymal tumors (38.90%), and hematopoietic tumors (4.14%) located in the skin. Among these, 69.25% were benign and 30.74% were malignant. The top ten most frequently diagnosed cutaneous tumors were epidermal and follicular cysts (12.70%), lipoma (11.36%), mast cell tumors (8.82%), cutaneous histiocytoma (7.49%), basal cell tumors (6.82%), sebaceous gland adenoma (6.68%), sebaceous gland hyperplasia (5.08%), hepatoid gland adenoma (3.61%), apocrine adenocarcinoma (3.07%), and fibroma (2.81%), in order of prevalence. They comprised 68.45% of all cutaneous tumors. These top ten cutaneous tumors were distributed on the trunk (30.08%), head and neck (20.9%), extremities (19.14%), anal and perianal area (8.59%), and tail (3.91%). The age of the dogs with the ten most frequent tumors had a mean age of 8.3 years, with a range of 2 months to 19 years. When all types of tumors were considered together in the entire population, there was no difference in incidence according to sex.

A 2-year-old intact female Golden Retriever presented due to rapidly progressing depression, ascites, dysuria, abdominal pain, and severe vaginal bleeding. At necropsy, the retroperitoneal space was expanded by multiple coalescing neoplastic nodules and the uterine wall was thickened with poorly defined neoplastic infiltrates. The urinary bladder was markedly thickened due to botryoid nodules exhibiting exophytic growth into the lumen. Metastases to lung, liver, kidney, and abdominal and thoracic lymph nodes were also noted. Microscopically, the genital tract and retroperitoneal masses were consistent with the alveolar subtype of rhabdomysarcoma, while the urinary bladder mass had characteristics of the embryonal subtype. Immunohistochemically, the neoplastic cells in all these tissue sites were intensely positive for desmin, sacromeric actin, and vimentin, while they were uniformly negative for cytokeratin and smooth muscle actin. Phosphotungstic acid hematoxylin stain revealed cross-striations in the cytoplasm of scattered neoplastic cells. Based on the gross findings, histopathology, and immunohistochemistry, genitourinary rhabdomyosarcoma with multisystemic metastases was made.

ABSTRACT. Pyelonephritis, in which Staphylococcus intermedius was isolated, was diagnosed in a 4-year-old female Siberian tiger (Panthera tigris altaica). At necropsy, the renal pelvis was dilated with a large amount of purulent exudates. Microscopically, the lesions of renal pelvis and medulla consisted of necrotic foci intermingled primarily with numerous degenerative neutrophils and a few lymphocytes, plasma cells and macrophages. Bacteriology showed the presence of S. intermedius. This is the first report on pyelonephritis associated with S. intermedius in wild felidae. KEY WORDS: Panthera tigris altaica, pyelonephritis, Staphylococcus intermedius.J. Vet. Med. Sci. 69(8): 851-852, 2007 Staphylococcus intermedius is a gram-positive, nonmotile, non-spore forming, facultative anaerobic coccus [3], which is part of the normal microflora of the skin and mucosal surfaces of the upper respiratory tract of dogs, horses, cats, and minks [1,7]. This bacterium has also been isolated from the anterior nares of dogs and pigeons [4], and from infected dog bite wounds in humans [10]. S. intermedius may cause cutaneous, urinary tract, bone, and central nervous system infections in several animal species [1,7].In dogs, S. intermedius is one of the leading pus-forming bacteria and causes infections such as pyoderma and otitis externa [3,8]. Staphylococcus spp. does not generally appear to cause any major disease in cats [5], but cases of superficial dermatitis, bacterial folliculitis, and superficial pyoderma caused by S. intermedius have been reported [9]. About 10% (9/93) of Staphylococci obtained from feline clinical specimens were S. intermedius [3,5].Diseases associated with S. intermedius have not been reported in wild felidae. Here we describe an episode of a Siberian tiger (Panthera tigris altaica) kept at the Korea National Arboretum with pyelonephritis caused by S. intermedius.The four-year-old female Siberian tiger had been donated from China for breeding and was raised for four months alongside a male Siberian tiger at the Korea National Arboretum. After showing depression and anorexia over several days, the female tiger suddenly died. A postmortem examination was performed immediately.At necropsy, the dead Siberian tiger was thin and emaciated. No wounds or disease conditions were observed in the external body surfaces and orifices, and the tiger was not pregnant. Both kidneys were congested, and the pelvis of the left kidney was markedly dilated and filled with a large amount of inspissated purulent exudates (Fig. 1). No purulent exudates or any significant gross abnormalities were found in the urinary bladder and ureter. Representative samples from major parenchymal tissues, including the heart, lungs, liver, spleen, kidneys, small and large intestines, urinary bladder, ovaries and uterus, were fixed in 10% neutral phosphate-buffered formalin, routinely processed, embedded in paraffin, and stained with hematoxylin and eosin (HE) for histopathologic examinations. The normal histologic architecture of the rena...

Multilobular tumor of bone detected in a 2.5-year-old male Pekingese dog is reported. Grossly, the neoplasm consisted of multiple, variably sized, gritty, grayish-white to yellow nodules separated by thick collagenous septa. Histologically, these nodules contained multiple lobules of irregularly shaped and sized islands of well-differentiated osteoid and cartilage, separated by anastomosing fibrovascular septa. Chondrocytes and osteocytes were observed in the lacunae and in more osseous islands in the lobule, respectively. These lobules were surrounded by mesenchymal spindle cells. Mitotic figures were not evident. The neoplastic pattern was consistent with that of a multilobular bone tumor. Diagnosis was based on gross and light microscopic findings. The cause of this neoplasm was not determined.

A 7-year-old female Eurasian otter (Lutra lutra) at the Seoul Grand Park, Korea, died after displaying depression, anorexia, weight loss and rough skin for several days. At necropsy, a solitary friable round mass, which was approximately 12 × 9 × 5 cm and mottled dark red and yellow, was found bulging from the right hepatic lobe. Microscopically, the nonencapsulated, poorly circumscribed mass was composed of solid sheets of neoplastic hepatocytes. In addition, numerous small tan foci, ranging from 0.5 to 1.0 cm in diameter, were evenly scattered throughout the pancreatic tissue. These foci were found to be nonencapsulated, well-demarcated hyperplastic nodules of the exocrine pancreatic gland. We observed neither intrahepatic nor extrahepatic metastases. Based on the gross and microscopic changes, we diagnosed the animal as having a hepatocellular adenoma accompanied by exocrine pancreatic nodular hyperplasia.