Abstract. It has been known that Arthropods Pheretima asperfillm have high medicinal value. The insect secondary metabolite and antibacterial substance of arthropods are the important research field in chemical ecology. In this paper, three compounds were separated and purified from Pheretima asperfillm, arthropods by silica gel column chromatography and semi-preparative high performance liquid chromatography. Then, three compounds were identified as Earthworm monoterpene ester (compound 1), 2-isopropyl flavanone (compound 2) and Mannitol(compound 3) and based on the spectral NMR analysis including 2DNMR.
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