A study was undertaken to develop an effective protocol to generate doubled haploid plants from a proprietary Mahyco hybrid MRP5401. A total of 20,300 anthers were plated on four different DH media (basal N6 + phytohormones) and obtained a callus induction success rate of 1.52%, of which 0.48% developed into green plants. A total of 232 lines were obtained of which, 98 lines showed homozygosity in DH 2-3 generations. Well established DH lines from MRP5401 in DH 3 generation had early flowering and yield gain.

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The present study was performed to generate double haploid lines in bell pepper with high regeneration efficiency via anther culture. In total, 825 anthers were plated on five different modified MS media viz., MS I, MS II, MS III, MS IV and MS V. The number of embryoids formed was found to be highest on MS IV media (26 embryoids) as compared to others. Forty five embryoids were developed on five media combinations, out of which 19 were regenerated into plantlets. Therefore, the optimized media i.e. MS IV in this study may be suitable for the development of double haploid lines in other economically important bell pepper as well as hot pepper genotypes through anther culture.

The present study was carried out with six wheat and four maize genotypes from Mahyco Research Centre, Dawalwadi, Jalna, MH to develop wheat double haploid lines by wheat x maize crossing system. The following steps were followed while conducting the experiment: emasculation of wheat spikes, crossing or pollination of emasculated spikes with maize pollens, treating pollinated spikes with 100 ppm 2,4-D solution, excision of embryo and subsequent culture on half strength MS media. In total, 296 spikes were emasculated and pollinated with randomly collected maize pollens after 24 hrs. Success was obtained in rescuing a total of 27 embryos from 575 caryopses among two genotypes i.e. MW2 and MW3. Percentage of embryo formation varied in the range of 0-10 per cent owing to non-standard conditions. However, the frequency of embryo formation could be increased by focusing on the most important of all culture conditions i.e. temperature (18-21 °C).