Introduction: This case report refers to 54 years old infertile woman who visit ACHARYA VINOBABHAVE RURAL HOSPITAL for her ARTHRITIS PROBLEM with her husband (age 61-year-old). During her treatment, she got to know about IVF/ART procedures. As she belongs to a rural area and is not so educated, she was not aware of this and start investigating it and found it interesting. After knowing about all these procedures, a new ray of hope was awakened in her that, she can also give birth to her baby after so much waiting and facing so much criticism by society as a barren woman. In the 36th week of gestation, one healthy baby girl has been delivered by Cesarean section. In this way, the faith between patient and doctor was built-up and inevitably, we can appreciate this case of postmenopausal gestation (Geriatric Pregnancy) As A Therapeutic Victory For Wardha Test Tube Baby Centre and for the patient as well. This infertile couple was not that financially strong and even this all procedure is too much unaffordable to them still after all these, their urge to having a baby is so high that, they invest financially as well as emotionally and also mother put her health in peril while going through IVF/ART procedure. Being an infertile woman, she has to face a lot, and in such conditions, women go through psychological torture, and from this depressive phase, many health issues arise. Main Symptoms and/or Important Clinical Findings: Menopausal woman, qualitative analysis of AMH show 0 ng/ml, means diminished ovarian reserve (DOR). The Main Diagnoses, Therapeutic Interventions, and Outcomes: The woman is pinpointed with primary infertility for 26 years. The couple suggested going for IVF/ICSI treatment with donor oocytes(frozen) and self-sperm sample, ICSI is performed and sequential embryo transfer is done and gets conceived in the first cycle of ART. As being a menopausal woman, the dose of oral intake of estrogen hormone tablets doubles as an HRT treatment. Conclusion: Intake of the double dose of estrogen orally (hormone replacement therapy, HRT) than the normal women whose age is not much and who is not menopausal, increases the endometrium thickness and its receptivity which helps the woman to conceive in the first cycle through ART.
Background: Folate is present in Vitamin B, which is not present in the body, and one has to consume it from outside. Its functions are to help in the production of RBCs, helps in the production of genes, DNA, RNA, and protein, helps in the metabolism of homocysteine, and is also the main component which is required for the process of methylation. For the metabolism of the folate group, the MTHFR gene is responsible. When there is a problem occurs in the MTHFR gene such as SNP, then it can create a problem with many allied metabolisms and other biological reactions or processes in the body of human body and deficiency of folate in the body may lead to affect various processes such as it can increase the homocysteine level in the body and can cause hyperhomocysteinemia, which has been linked with many diseases along with causing male infertility. Objectives: To identify nucleotide polymorphism in cases and control group. To correlate polymorphism status with male infertility. To correlate MTHFR polymorphism with abnormal semen parameters. To correlate BMI with male infertility. Methodology: For this study, we will use various collective methods to conduct the study which includes the recording of treatment history and the indications of infertile patients visiting WARDHA TEST TUBE CENTRE and counselling them. After that semen collection and analysis will be done along with blood collection, DNA extraction, DNA quantification with nanodrop and agarose gel, PCR, and RFLP. We have also made use of PubMed for getting related articles. Results and Conclusion: We have done many searches on PubMed and NCBI sites (http://www.ncbi.nlm.nih.gov/pubmed/) with keyword MTHFR gene, polymorphism in MTHFR gene, methylation, folate methylation, methylenetetrahydrofolate reductase, methylenetetrahydrofolate reductase, and primer for MTHFR gene and retrieved many articles related to primers for MTHFR gene and will design our primer for forward and reverse annealing with the help of Amplify software (Engels, 1993). With the help of our study, we will analyze the MTHFR genomic sequence along with analysis of RFLP, in which we will design an amplicon of 513bps in which we will find restriction site for Hinf I and will check that if polymorphism is there then it will cut the DNA amplicon into two and will confirm it with electrophoresis.
The grounds of the contemporaneous examination is to evaluate the association in unite repercussions of mind-body intercession and serum cortisol measures and eggs attributes. The cognizance that psychical stress may avert females from accomplishing and keeping up a gestation has become vastly received. Mind-body intercession incorporates calmness, optimistic make-believe, biofeedback, balm, meditation, rumination, yoga, and more. All these acts work discreetly to help someone alleviate illness. Mind-body interventions (MBIs) may be an efficacious implement to assist females to cope with the ultimatum of infertility pinpointing and ministration Objective of this study is to sensitize all sub fertile females about the study, to investigate serum cortisol level of sub fertile females and to investigate the quality of oocyte of sub fertile females. This non-invasive methodology appends a set down of ministration history and the manifestations. Advice will be given to all the patients for research. Additional convention appends checking of oocytes quality after mind-body intercession in the second cycle of IVF after retrieving poor quality of oocytes in the first cycle and failure of the cycle due to it.
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