The demand for nutritious food, especially food of animal origin, is globally increasing due to escalating population growth and a dietary shift to animal source food. In order to fulfill the requirements, producers are using veterinary drugs such as hormones and hormone-like anabolic agents. Hormones such as steroidal (estrogens, gestagens, and androgens), nonsteroidal, semisynthetic, and synthetic or designer drugs are all growth-promoting and body-partitioning agents. Hence, in food animal production practice, farm owners use these chemicals to improve body weight gain, increase feed conversion efficiency, and productivity. However, the use of these hormones and hormonal growth-promoting agents eventually ends up with the occurrence of residues in the animal-originated food. The incidence of hormone residues in such types of food and food products beyond the tolerance acts as a risk factor for the occurrence of potential public health problems. Currently, different international and national regulatory bodies have placed requirements and legislative frameworks, which enable them to implement residue monitoring test endeavors that safeguard the public and facilitate the trading activity. To make the tests on the animal-origin food matrix, there are different sample extraction techniques such as accelerated solvent extraction, supercritical fluid extraction, solid phase extraction, solid-phase microextraction, and hollow-fiber liquid-phase microextraction. After sample preparation steps, the analytes of interest can be assayed by screening and confirmatory methods of analysis. For screening, immunological tests such as ELISA and radioimmunoassay are used. Detection and determination of the specific residues will be done by chromatographic or instrumental analysis. Mainly, among high-performance liquid chromatography, liquid chromatography with mass spectrometry (LC-MS, LC-MS/MS), and gas chromatography with mass spectrometry (GC-MS and GC-MS/MS) methods, LC-MS/MS is being preferred because of easier sample preparation without a derivatization step and high detection and quantification capacity.
