Many publications have dealt with the de novo biosynthesis of the methionine methyl group by animal systems in vitro( 1-S), but no work has been reported on the organ and intracellular location of this biosynthetic system. This is the purpose of this communication.Procedures. To determine which organs were the most active in synthesizing methionine methyl groups, 2 rats were used in each of 3 experiments. The brain, pectoralis * This work was supported in part by a PHS grant 2.G-627 to P. Gyorgy, M. D. muscles, kidneys, and liver were removed immediately upon killing. The tissues were rinsed, patted dry, weighed, and homogenized in a Servall "Omni-mixer" at full speed at 0°C for 2 minutes with 4 ml of 0.05 M phosphate buffer (pH 7.2) per g of tissue. The homogenates were then centrifuged at ca. 25,000 X g for 3 hours at 0°C and the supernatants used in the assays.In the investigations on the intracellular location, the liver was used because of results obtained in the first part of this study, a single rat being used per experiment. After
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