No abstract

To estimate the incidence of Dirofilaria immitis in Austrian shelter dogs and mosquitoes trapped in their proximity, 115 shelter dogs from fourteen animal shelters located in five different Austrian states were examined. Blood samples were screened for D. immitis using ELISA antigen-testing, PCR and microscopical examination for microfilariae. In total, 91% of the dogs originated from countries endemic for dirofilariosis. Eleven dogs (9.6%), all originating from Hungary, tested positive for D. immitis. None of the dogs examined showed microfilaremia. Eight dogs showed no or only mild clinical signs (e.g., infrequent coughing), and three dogs showed frequent coughing, dyspnea, exercise intolerance, blunt fur or weight loss. In total, 205 Mosquitoes of ten different species were caught at five different shelter sites in four different Austrian states, using CO2-baited mosquito traps set once a month (June–September 2019) for 24 h. All 205 mosquitoes tested negative for Dirofilaria spp. via PCR. The risk of endemisation of D. immitis in Austria (and other non-endemic countries in a similar situation) is very serious and its zoonotic potential should be communicated more strongly. To monitor a possible transmission of microfilariae from untreated or even untested positive dogs, e.g., in animal shelters, to mosquitoes in the near surroundings, frequent screening for Dirofilaria in mosquitoes should be used more intensively. Current knowledge on D. immitis should be integrated into daily veterinary practice and dog owners should be proactively educated, especially before traveling to endemic areas or adopting dogs from endemic countries. Animal shelters and animal welfare organizations should be provided with appropriate education and veterinary guidance regarding the testing and treatment of dogs imported from high-risk areas.

Vector-borne diseases play a major role in human and veterinary medicine worldwide. A previous study detected asymptomatic vector-borne pathogens in military working dogs stationed at a military base in eastern Austria, and a follow-up survey of potential arthropod vectors was conducted in spring 2019 and 2020 in the vicinity of the base to evaluate the presence of vectors and their carrier status for a range of canine and zoonotic pathogens. A total of 1324 ticks (nymphs and adults of Ixodes ricinus, comprising 92.9% of the collected specimens, and adults of Haemaphysalis inermis, a tick previously only rarely described in Austria, Haemaphysalis concinna, and Dermacentor reticulatus) were collected by flagging. In 44.1% (125/284) of all pools (n = 284), one infectious agent was found; in 27.8% (79/284) and in 1.1% (3/284), two and three different agents, respectively, could be identified. Overall, 72.9% of the pools contained at least one pathogen (Borrelia spp., Rickettsia spp., Bartonella spp., and Babesia microti). Borrelia mijamotoi, B. lustinaniae, and B. microti were previously only described in single cases in Austria. Mosquitoes were collected with BG-Sentinel traps monthly during the summer of 2019. A total of 71 individuals from 11 species were collected. No filarioid DNA was detected in the mosquito sample pools, although Dirofilaria repens had been present in the dogs from the military site. In conclusion, vector surveillance should be combined with the surveillance of an exposed population whenever possible to estimate the infection risks for dogs and their handlers.