SummaryHamster platelets have been separated from their plasmatic enviroment by means of gel filtration. This method of separation gave an excellent yield of platelets free of non-adsorbed plasma proteins.Gel filtered platelets (GFP), and platelets washed by repeated centrifugations and resuspensions (CP) were compared to each other and to platelets left undisturbed in their native plasma (PRP). Only slight morphological differences between the three platelet preparations could be detected by phase microscopy. In the concentration range of .28–.71 μM ADP, GFP, resuspended in PPP, showed the same degree of aggregation as did PRP. At lower concentrations of ADP, GFP aggregated to a lesser degree than did PRP. CP exhibited aggregation comparable to PRP only at higher concentrations of ADP (.71–1.42 (μM ADP). GFP also supported clot retraction to the same extent as did PRP.It would appear that gel filtration provides a new, gentle and rapid method for separation, from plasma, of platelets with activity very similar to that of platelets in PRP.
The red blood cells of Mr. R. W., an 86‐year‐old type O patient with Proteus mirabilis wound infection, anemia, and thrombocytopenia were found to be polyagglutinable due to Tn‐activation, to be agglutinated by and to absorb anti‐A, and to have a reduced sialic acid level probably as a result of enzyme modification in vivo. The reaction with anti‐A was inhibited by blood group A substance. It is possible that both Tn‐activation and acquisition of A‐like antigen were changes induced by Proteus infection but, if so, the changes persisted after the bacterial infection was cured. The red blood cell modification in this patient has features in common with others in whom T‐ or Tn‐activation, reduced sialic acid levels, or acquired B antigens have been found. Various abnormalities of the red blood cell membrane are discussed and clinical, serological, and physicochemical findings are compared.
T‐ and Tn‐activation are known to result in polyagglutinability of red blood cells. Unlike polyagglutinability associated with the presence of the Cad antigen, there is no association in these two states with the antigen Sda. Alteration or destruction of the T‐receptor on T‐activated cells by the proteases fidn and papain is strictly dependent on the strength of the protease solution used. There is no correlation of amounts of anti‐T and anti‐Tn in normal sera.
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