The ability of Co^2+ to substitute for Mn^2+ in exogenous and endogenous galactosyltransférase
reactions was tested. Exogenous transfer was measured towards different
high and low molecular weight galactose acceptors using galactosyltransférase from the following
sources: crude serum, the serum enzyme partially purified by affinity chromatography
and a pure enzyme preparation from milk. Endogenous transfer was estimated in preparations
from human urinary bladder tumor cells and from rat liver microsomal fractions.
The results show that Co^2+ is able to substitute for Mn^2+ in some exogenous and endogenous
galactosyltransférase reactions. This ability seems to depend on the molecular structure of
the galactose acceptor as well as on the nature of the enzyme.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.