This work deals with a standardized modification of estimating prothrombin concentration (or activity). It places especial emphasis upon the difference between the prothrombin times of whole and diluted plasmas, taking into consideration the respective absolute values of these readings. By these 3 measurements it appears possible to detect certain abnormalities of blood not revealed by the prothrombin time of whole or diluted plasma considered alone.The, technic used for the estimation of prothrombin was that described by Link and his coworkers' based on the method of Quick, Stanley-Brown, and BancrofL2 In the present study two series of estimations were made : one using the thromboplastin-calcium chloride mixture of Link and his students' and the other using Russell snake-viper venom* 3 7 4.5 nil freshly drawn venous blood were added to 0.5 ml M/10 sodium oxalate. Clear plasma was obtained by centrifuging. 0.1 ml whole plasma which had been kept at 37°C was quickly added to 0.2 ml thromboplastin-calcium chloride mixture (also at 37°C) and the time which elapsed before the fibrin clot formed was noted with a stop-watch. The identical procedure was then repeated using 0.1 in1 of 12.5 % plasma in place of whole plasma.When venom was substituted for the thrornboplastin the technic was as follows : 0.1 ml whole plasma was added to 0.1 iml venom and placed in the constant temperature bath at 37°C for 5-10 minutes. To this was quickly added 0.1 ml M/40 calcium chloride which had been kept in place of rabbit brain extract. Procedure.
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