Purpose: Hyperspectral imaging (HSI) is a non-contact optical imaging technique with the potential to serve as an intraoperative computer-aided diagnostic tool. Our work analyzes the optical properties of visible structures in the surgical field for automatic tissue categorization. Approach: Building an HSI-based computer-aided tissue analysis system requires accurate ground truth and validation of optical soft tissue properties as these show large variability. We introduce and validate two different hyperspectral intraoperative imaging setups and their use for the analysis of optical tissue properties. First, we present an improved multispectral filterwheel setup integrated into a fully digital microscope. Second, we present a novel setup of two hyperspectral snapshot cameras for intraoperative usage. Both setups are operating in the spectral range of 400 up to 975 nm. They are calibrated and validated using the same database and calibration set. Results: For validation, a color chart with 18 well-defined color spectra in the visual range is analyzed. Thus the results acquired with both settings become transferable and comparable to each other as well as between different interventions. On patient data of two different otorhinolaryngology procedures, we analyze the optical behaviors of different soft tissues and show a visualization of such different spectral information. Conclusion: The introduced calibration pipeline for different HSI setups allows comparison between all acquired spectral information. Clinical in vivo data underline the potential of HSI as an intraoperative diagnostic tool and the clinical usability of both introduced setups. Thereby, we demonstrate their feasibility for the in vivo analysis and categorization of different human soft tissues.
The extraction of heart rate and other vital parameters from video recordings of a person has attracted much attention over the last years. In this paper, we examine time differences between distinct spatial regions using remote photoplethysmography (rPPG) in order to extract the blood flow path through human skin tissue in the neck and face. We can show that the visualization of the blood flow path corresponds to the physiologically defined path.
Flap loss through limited perfusion remains a major complication in reconstructive surgery. Continuous monitoring of perfusion will facilitate early detection of insufficient perfusion. Remote or imaging photoplethysmography (rPPG/iPPG) as a non-contact, non-ionizing, and non-invasive monitoring technique provides objective and reproducible information on physiological parameters. The aim of this study is to establish rPPG for intra- and postoperative monitoring of flap perfusion in patients undergoing reconstruction with free fasciocutaneous flaps (FFCF). We developed a monitoring algorithm for flap perfusion, which was evaluated in 15 patients. For 14 patients, ischemia of the FFCF in the forearm and successful reperfusion of the implanted FFCF was quantified based on the local signal. One FFCF showed no perfusion after reperfusion and devitalized in the course. Intraoperative monitoring of perfusion with rPPG provides objective and reproducible results. Therefore, rPPG is a promising technology for standard flap perfusion monitoring on low costs without the need for additional monitoring devices.
This paper presents an approach to assess the perfusion of visible human tissue from RGB video files. We propose metrics derived from remote photoplethysmography (rPPG) signals to detect whether a tissue is adequately supplied with blood. The perfusion analysis is done in three different scales, offering a flexible approach for different applications. We perform a plane-orthogonal-to-skin rPPG independently for locally defined regions of interest on each scale. From the extracted signals, we derive the signalto-noise ratio, magnitude in the frequency domain, heart rate, perfusion index as well as correlation between specific rPPG signals in order to locally assess the perfusion of a specific region of human tissue. We show that locally resolved rPPG has a broad range of applications. As exemplary applications, we present results in intraoperative perfusion analysis and visualization during skin and organ transplantation as well as an application for liveliness assessment for the detection of presentation attacks to authentication systems.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.