Background Short-sprint (≤ 20 m) performance is an important quality for success in the football codes. Therefore, developing an evidence base for understanding training methods to enhance short-sprint performance is key for practitioners. However, current systematic reviews are limited by (1) a lack of focus on football code athletes, (2) a lack of consideration of all training modalities and (3) a failure to account for the normal training practices undertaken by intervention groups within their analysis. Therefore, this review aimed to (1) conduct a systematic review of the scientific literature evaluating training interventions upon short-sprint performance within football code athletes, (2) undertake a meta-analysis to assess the magnitude of change of sport-sprint performance following training interventions and (3) identify how moderator variables affect the training response. Methods A systematic search of electronic databases was conducted. A random-effects meta-analysis was performed to establish standardised mean difference with 95% confidence intervals. This identified the magnitude and direction of the individual training effects of intervention subgroups (primary, secondary, combined-specific, tertiary and combined training methods) on short-sprint performance while considering moderator variables (i.e., football code, sex, age, playing standard, phase of season). Results 121 studies met the inclusion criteria, totalling 3419 athletes. Significant improvements (small-large) were found between pre- and post-training in short-sprint performance for the combined, secondary, tertiary and combined-specific training methods. No significant effect was found for primary or sport only training. No individual mode was found to be the most effective. Between-subgroup analysis identified that football code, age, playing standard and phase of season all moderated the overall magnitude of training effects. Conclusions This review provides the largest systematic review and meta-analysis of short-sprint performance development methods and the only one to assess football code athletes exclusively. Practitioners can apply combined, secondary and tertiary training methods to improve short-sprint performance within football code athletes. The application of sport only and primary methods does not appear to improve short-sprint performance. Regardless of the population characteristics, short-sprint performance can be enhanced by increasing either or both the magnitude and the orientation of force an athlete can generate in the sprinting action. Trial Registration OSF registration https://osf.io/kshqn/.
Female sports have recently seen a dramatic rise in participation and professionalism world-wide. Despite progress, the infrastructure and general sport science provisions in many female sports are behind their male counterparts. From a performance perspective, marked differences in physical and physiological characteristics can be seen between the sexes. Although physical preparation practices for male athletes are known, there are currently no published literature pertaining exclusively to female athletes. This information would provide invaluable data for both the researcher and practitioner alike. This survey therefore aimed to examine current practices utilized in female rugby codes (union, league, and sevens). A questionnaire assessing seasonal physical preparation practices, recovery, monitoring and sport science technology, and unique aspects in female rugby was developed. Thirty-seven physical preparation practitioners (32 males, 5 females) responded to the questionnaire. Most participants (78%) worked with national or regional/state level female athletes. Performance testing was more frequently assessed in the pre- (97%) and in-season (86%), than off-season (23%). Resistance, cardiovascular, sprint and plyometric training, and recovery sessions were all believed to be important to enhancing performance and implemented by most participants (≥ 89%). Sport science technologies were commonly (54%) utilized to inform current practice. Menstrual cycle phase was monitored by 22% of practitioners. The most frequently reported unique considerations in female rugby codes included psycho-social aspects (41%), the menstrual cycle (22%), and physical differences (22%). Practitioners working with female rugby can use the presented data to inform and develop current practices.
To facilitate the study of the mechanisms of breast cancer metastasis we have previously characterized a pair of breast tumor cell lines that originate from the same breast tumor cell line MDA-MB-435, but which have diametrically opposite metastatic capabilities. These cell lines constitute a stable and accessible experimental system for the identification of metastasisrelated genes and for the study of their role in the process of metastasis. In this study, we used a combination of RNA arbitrarily primed-polymerase chain reaction (RAP-PCR) fingerprinting and cDNA arrays (here termed "RAP-array") to identify genes differentially expressed with respect to metastatic phenotype. The multistep nature of metastasis poses difficulties in both design and interpretation of experiments to unveil the mechanisms causing the process. To facilitate such studies we have recently developed and characterized an isogenic cell line model.1 By serial dilution cloning of the MDA-MB-435 breast tumor cell line and screening by orthotopic implantation into the mammary fat pad of athymic mice, a pair of breast tumor cell lines (M-4A4 and NM-2C5) that originate from the same breast tumor, but that have diametrically opposite metastatic capabilities were derived. In 74% of inoculated athymic mice clone M-4A4 metastasized consistently to the lungs and the lymph nodes, mimicking major dissemination routes of human breast cancer. Conversely, although equally tumorigenic, clone NM-2C5 did not metastasize to any distal site. We have confirmed that the cell lines originate from a single genetic source by spectral karyotyping and evaluated the expression of a number of proteins previously implicated in cellular transformation and metastasis. Initial targeted and exploratory RNA and protein analyses have revealed that the secreted proteins thrombospondin-1 and osteopontin are differentially expressed. 1 Subsequently, representational difference analysis of cDNA obtained from the two clonal populations revealed a correlation between the increased expression of tyrosinase-related protein-1 and the matrix metalloproteinase-8 genes with the nonmetastatic phenotype.2 These cell lines constitute a stable and accessible model for the identification of genes involved in the multistep process of breast tumor metastasis.Modern technological advances now permit the application of high-throughput gene expression analysis to identify patterns of expression involved in complex biological processes. While the field of gene expression
Background Within the football codes, medium-distance (i.e., > 20 m and ≤ 40 m) and long-distance (i.e., > 40 m) sprint performance and maximum velocity sprinting are important capacities for success. Despite this, no research has identified the most effective training methods for enhancing medium- to long-distance sprint outcomes. Objectives This systematic review with meta-analysis aimed to (1) analyse the ability of different methods to enhance medium- to long-distance sprint performance outcomes (0–30 m, 0 to > 30 m, and the maximum sprinting velocity phase [Vmax]) within football code athletes and (2) identify how moderator variables (i.e., football code, sex, age, playing standard, phase of season) affected the training response. Methods We conducted a systematic search of electronic databases and performed a random-effects meta-analysis (within-group changes and pairwise between-group differences) to establish standardised mean differences (SMDs) with 95% confidence intervals and 95% prediction intervals. This identified the magnitude and direction of the individual training effects of intervention subgroups (sport only; primary, secondary, tertiary, and combined training methods) on medium- to long-distance sprint performance while considering moderator variables. Results In total, 60 studies met the inclusion criteria (26 with a sport-only control group), totalling 111 intervention groups and 1500 athletes. The within-group changes design reported significant performance improvements (small–moderate) between pre- and post-training for the combined, secondary (0–30 and 0 to > 30 m), and tertiary training methods (0–30 m). A significant moderate improvement was found in the Vmax phase performance only for tertiary training methods, with no significant effect found for sport only or primary training methods. The pairwise between-group differences design (experimental vs. control) reported favourable performance improvements (large SMD) for the combined (0 to > 30 m), primary (Vmax phase), secondary (0–30 m), and tertiary methods (all outcomes) when compared with the sport-only control groups. Subgroup analysis showed that the significant differences between the meta-analysis designs consistently demonstrated a larger effect in the pairwise between-group differences than the within-group change. No individual training mode was found to be the most effective. Subgroup analysis identified that football code, age, and phase of season moderated the overall magnitude of training effects. Conclusions This review provides the first systematic review and meta-analysis of all sprint performance development methods exclusively in football code athletes. Secondary, tertiary, and combined training methods appeared to improve medium-long sprint performance of football code athletes. Tertiary training methods should be implemented to enhance Vmax phase performance. Nether sport-only nor primary training methods appeared to enhance medium to long sprint performance. Performance changes may be attributed to either adaptations specific to the acceleration or Vmax phases, or both, but not exclusively Vmax. Regardless of the population characteristics, sprint performance can be enhanced by increasing either the magnitude or the orientation of force an athlete can generate in the sprinting action, or both. Trial Registration OSF registration https://osf.io/kshqn/.
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