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Anaerobic bacteria were identified in live drilling muds and cores from nine different North Sea and Irish Basin oilfields, varying in depth from 3500 to 15000 ft, and at temperatures up to 150°C. The anaerobic bacteria may be introduced into the reservoir during drilling operations or injection of water, but in many cases the bacteria are indigenous to the oilfield reservoirs. Confirmation of the indigenous anaerobic bacteria was made using molecular biology techniques (16S rDNA sequence analysis), comparing microbial populations present in the blank drilling mud as supplied to wellsite, in the live drilling mud taken during coring, and in the live core. The role of anaerobic bacteria in oilfield diagenesis is not fully understood, though pyrite precipitation, and exopolymer and H2S gas production were noted in this study, up to temperatures of 95°C.

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Abstract. Antarctic continental ice masses fluctuated considerably in size during the elevated atmospheric CO2 concentrations (~ 600–800 ppm) of the Oligocene “coolhouse”. To evaluate the role of ocean conditions to the Oligocene ice sheet variability requires understanding of past ocean conditions around the ice sheet. While warm ocean conditions have been reconstructed for the Oligocene Wilkes Land region, questions arise on the geographical extent of that warmth. Currently, we lack data on surface ocean conditions from circum-Antarctic locations, and ice-proximal to ice-distal temperature gradients are poorly documented. In this study, we reconstruct past surface ocean conditions from glaciomarine sediments recovered from the Deep Sea Drilling Project (DSDP) Site 274, offshore the Ross Sea continental margin. This site offshore Cape Adare is ideally located to characterise the Oligocene regional surface ocean conditions, as it is situated between the colder, ice-proximal Ross Sea continental shelf, and the warm-temperate Wilkes Land Margin in the Oligocene. We improve the existing age model of DSDP Site 274 using integrated bio- and magnetostratigraphy. Subsequently, we analyse dinoflagellate cyst assemblages and lipid biomarkers (TEX86) to reconstruct surface paleoceanographic conditions during the Oligocene (33.7–25.4 Ma). Both TEX86-based sea surface temperature (SST) and microplankton results show temperate (10–17 °C ± 5.2 °C) surface ocean conditions at Site 274 throughout the Oligocene. Increasingly similar oceanographic conditions between offshore Wilkes Land margin and Cape Adare developed towards the late Oligocene (26.5–25.4 Ma), likely in consequence of the widening of the Tasmanian Gateway, which resulted in more interconnected ocean basins and frontal systems. To maintain marine terminations of terrestrial ice sheets in a proto-Ross Sea with as warm offshore SST as our data suggests, requires a strong ice flux fed by intensive precipitation during colder orbital states in the Antarctic hinterland, but with extensive surface melt of terrestrial ice during warmer orbital states.