A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : This study aimed to investigate the effects of anti-NGF administration on apoptosis in rats with experimentally induced sepsis model, and to examine the possible role of nerve growth factor (NGF) in pathogenesis and treatment of sepsis. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Thirty three Wistar Albino rats were randomly allocated into four groups. After sparing the control group, the remaining three groups were given intraperitoneal E. coli lipopolysaccaride to induce sepsis. Early anti-NGF group (Group EA-NGF, n=9) was injected with intraperitoneal anti-NGF one hour before LPS injection, and late anti-NGF group (Group LA-NGF, n=9) after sepsis was induced. Baselines, 2, 12, 24, and 72nd hours were analyzed to determine NGF levels. The rats were sacrificed on the 3rd day, and tissue samples separated from the small intestines, liver and lung. HE, bcl-2 and bax staining were used to evaluate apoptosis. R Re es su ul lt ts s: : Apoptosis increased most markedly in the intestine tissue, as well as in the liver and lung tissue, and this increase was most evident in the early anti-NGF group. Bcl-2 staining in the subjects was less apparent while bax staining was more intensely detected in the sepsis groups, with a significant difference between the sepsis and early anti-NGF groups. Bax staining in the EA-NGF group was more clearly observed than that in LA-NGF group. NGF levels were found to increase in the 24th hour in sepsis group (Group S) and decrease in the second hour in Group EA-NGF. C Co on nc cl lu us si io on n: : It can be concluded that anti-NGF administration in the early period of sepsis increases apoptosis at least to the same extent with sepsis, and it decreases apoptosis when administered in the later period of sepsis.K Ke ey y W Wo or rd ds s: : Sepsis; 7S nerve growth factor protein, mouse; apoptosis Ö ÖZ ZE ET T A Am ma aç ç: : Bu ça lış ma da de ney sel sep sis mo de li oluş tu ru lan rat lar da, an ti-NGF uy gu lan ma sı nın apo pi toz üze ri ne et ki le ri ni de ğer len di re rek Ner ve growth fak tö rün (NGF) sep sis pa to ge nez ve teda vi sin de ki ola sı ro lü nün araş tı rıl ma sı amaç lan dı. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Otuz üç ta ne Wis tar-Al bino cin si rat rast ge le dört gru ba ay rıl dı. Kon trol gru bu ay rıl dık tan son ra di ğer üç gru ba E. co li lipopolisakkaridi in tra pe ri to ne al ola rak uy gu la na rak sep sis kli ni ği oluş tu rul du. Fark lı ola rak er ken an ti-NGF grup (Grup EA-NGF, n=9) LPS uy gu lan ma dan 1 sa at ön ce ve geç an ti-NGF grup (Grup LA-NGF, n=9 )'ye sep sis kli ni ği oluş tu rul duk tan son ra an ti-NGF in tra pe ri to ne al ola rak uy gu lan dı. De nek ler den ba zal, 2, 12, 24 ve 72. sa at ler de kan ör nek le ri alı na rak se rum NGF dü zey le ri öl çül dü. De ne yin üçüncü gü nün de sak ri fi ye edi len rat la rın in ce bar sak, ka ra ci ğer ve ak ci ğer do ku la rı ay rı -la rak he ma tok si len eo zin (H/E) ve im mu no his to kim ya sal ola ...
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