The aim of this study was to evaluate the effect of probiotic oligosaccharides (POS) on the recovery of bowel atrophy induced by protein malnutrition (PM) in rats. Thirty male Wistar rats (200 to 250 g) were fed with a conventional diet for 10 days of adaptation. After this period, the rats are divided into 3 groups. A control group was fed with a conventional diet (n = 10), and a group of 20 rats was subjected to PM for 15 days. After period of PM, 10 rats were refed with enriched POS diet for 10 days (Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium bifidum, Streptococcus thermophilus and fructo-oligosaccharides at 0.5 mg/g body weight/day). Our experimental protocol was developed to evaluate some nutritional biochemical parameters such as total proteins, globulin and albumin and morphometric parameters of the intestine. Biochemical parameters caused a significant decrease in the concentration of total protein, albumin and globulin in malnourished rats as compared between the control and group rats. These biochemical values increased in rats refed with POS. The data obtained in the study suggest that PM causes alterations in rat colon and small intestinal morphometry, especially in tissues which present a high level of cell turnover such as the mucosa tunic and consequently their structures such as the enterocytes, goblet cells and crypts. Probiotics oligosaccharides enhanced the recovery of gut atrophy induced by protein malnutrition. Probiotic oligosaccharides can be useful as oral adjuvant during the recovery of protein malnutrition.
The botanic genera Pistacia are groups around een species of shrubs that belong to the Anacardiaceae family which is believed to have originated in Asian or Mediterranean region. P. len scus is abundant species of the Pistacia genus encountered in the forest region of Ouarsenis mount (Northwest of Algeria), exactly in the na onal park of Thniet El Had in the Wilaya of Tissemsilt in Algeria. In the present study, chemical composi on and an bac- terial ac vi es of essential oil (EO) of P. lentiscus were evaluated. The EO was isolated and analyzed by gas chromatography-mass spectrometry (GC- MS). The minimum inhibitory concentra on (MIC) was determined using 6 strains of Gram-positive and negative bacteria. The antibiogram was made following the gliosis-environment diffusion method and which makes it possible to determinate bacteria’s sensibility to an bio cs. From a exponential culture (18 to 24 hours), a bacterial suspension was prepared and well- homogenized a erwards. The opacity was adjusted equivalent to 0.5 McF (McFarland). Based on the GC-MS analysis, thirty five compounds representing 100 % of the total oil composi on were iden ed. The essen al oils could be explored to test their an microbial activity, especially against some bacteria that cause alimentary intoxications. The results revealed that the essen al oil exhibited strong levels of an bacterial activity against the tested microorganism regarding the MIC values, Salmonella sp was found to be the most sensitive strain (inhibition zone 23cm MIC 1.25%). Based on the findings of the present study, new an bacterial agents could be developed, and the use of P. lentiscus should be promoted in the traditional treatment of ailments.
The nutritional imbalance for the plant is caused mainly by the antagonisms between Potassium (K+), Calcium (Ca2+) and Magnesium (Mg2+). To ovoid this maladjustment, the concentrations of these elements must obey to K/(Ca + Mg) (R). This experiment was aimed at assessing the different responses of Fragaria ananassa (FA) soilless crop to the different Ca2+ concentrations (mM) : 0, 1.5, 3, 4.5, 6 and 9. They corresponding to different R: 2.5 1.42, 1, 0.76, 0.62, 0.45. It found that 0.62≤ R ≤ 0.76 provided relatively the best growths by improving the height plant, length, and width of the leaves, length of the petiole. It provided, also, the best biomass by the highest dry and fresh weight (DW, FW) with a correlation R2 = 0.37 and R2= 078 respec-tively. The greatest rate of H2PO4-, K+, Ca2+, Mg2+, Fe, Mn and Cu in leaves was , also, obtained by the same R. However, total Nitrogen (T-N) was not affected by the R variation. This variation on Ca2+ concentration in the sap of the petiole and on the aerial part was correlated R2 = 0.86 and 0.85 respec-tively. The electrical conductivity (EC) and the pH obtained under The R cited were around 1.2 dS/m and 6.25 respectively for a correlation R2=0.64 for pH.
The genera Cistus present groups of about twenty species of shrubs belon- ging to the Cistaceae family are very common throughout the Mediterranean region. Cistus salviifolius, known as Melliya is a species of this genus that occurs in the forest region of Mount Ouarsenis (northwest Algeria), exactly in Thniet El Had Na onal Park in the Wilaya of Tissemsilt. This study was un- dertaken to evaluate the an bacterial effects of C. salviifolius and its chemical composition in addition the effect of shade drying me on essen al oil yield in order to op mize this parameter which is most o en not controlled for producers. The op mal yield recorded is about 0.07± 0.02%. Its characte- riza on by gas chromatography coupled with mass spectrometry shows a dominance of hydrocarbon compounds. The minimum inhibitory concentration (MIC) is established using 6 strains of Gram-positive and negative bacteria. The antibiotic susceptibility test is performed using the giliose- environment diffusion method, a bacterial suspension is prepared and subsequently well homogenized. Opacity must be adjusted to 0.5 McF (McFarland), from a young exponen al culture (18 to 24 hours). The an mi- crobial ac vity of C. salviifolius EO was tested against three Gram-posi ve and three Gram-nega ve bacteria. In fact, a strong inhibitory and bactericidal effect has been demonstrated against all Gram-positive and Gram- negative bacteria tested. It appears from this study that the EO of C. salviifo- lius has a very high antimicrobial activity, it shows a wide spectrum covering Gram positive and Gram negative bacteria. Taking into account the results of this study, new an bacterial agents could be developed and the use of C.salviifolius should be encouraged in the pharmaceutical and perfumery industries.
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