We developed an in vitro DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase. Luminescence was induced upon colocalization of the reporter pair to a ∼44 bp target sequence defined by sgRNAs. We used the system to detect Mycobacterium tuberculosis DNA with high specificity and sensitivity. The reprogrammability of dCas9 was further leveraged in an array design that accesses sequence information across the entire genome.
The active and well-designed Schiff base ligands are considered "privileged ligands". The so-called salen ligands, i.e., the tetradentate [O, N, N, O] bis-Schiff base ligands, have also found broad applications in many homogeneous catalytic reactions. Modification of the salen ligands has concentrated on altering the substituents in the phenolate rings and variations in the diamine backbones. Herein, o-carborane-supported salen ligands (2) were designed and prepared. A series of aluminum−salen complexes (3• (sol) 2 ), which were supported by the nido-C 2 B 9 carborane anions, were synthesized. These Al(III) complexes showed high activities (TOF up to 1500 h −1 ) in catalyzing the cycloaddition of epoxides and CO 2 at atmospheric pressure and near room temperature. Complexes 3•(sol) 2 are one of the rare examples of Al-based catalysts capable of promoting cycloaddition at 1 bar pressure of CO 2 . Density functional theory (DFT) studies combined with the catalytic results reveal that the catalytic cycles occur on two axial sites of the Al(III) center.
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