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Abstract. The aim of the present study was to assess miR-625 and Fas expression in normal and degenerative cervical cartilage endplate (CEP) tissues. Following biof-informatics analysis, the Fas gene was predicted to be one of the targets of miR-625. Quantitative PCR (qPCR) and western blotting were used to detect miR-625 and Fas expression in normal and degenerative CEP. A luciferase reporter assay was used to identify whether miR-625 could directly target the 3' untranslated region (3'-UTR) of Fas. Lentiviral overexpression and/or inhibition vectors of miR-625 (pre-miR-625)/antigomiR-625 were constructed to determine whether overexpression or inhibition of miR-625 could affect Fas and B-cell lymphoma 2 (Bcl-2) expression in cartilaginous endplate cells (CECs) and tissues. qPCR analysis demonstrated that miR-625 expression in degenerative CEP was significantly lower than in normal CEP tissue, while the production of Fas in degenerated CEP was significantly higher. Results from western blotting also showed a significant increase in Fas expression in degenerative CEP. miR-625 can bind directly to the 3'-UTR of the Fas gene. However, this inhibition was attenuated by a target mutation in the miR-625-binding site of the 3'-UTR of Fas mRNA. In addition, following transfection of CECs with pre-miR-625 and antigomiR-625, expression of Fas significantly decreased and increased, respectively, and Bcl-2 expression was upregulated and downregulated, respectively. Upregulation of miR-625 can inhibit Fas expression and further affect Bcl-2 expression in CEP degeneration, suggesting that miR-625-mediated inhibition of the Fas gene is important in cervical degeneration.

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On May 22, 2021, a 7.4 magnitude earthquake struck Maduo County (34.59°N, 98.34°E), Qinghai Province, China, with a focal depth of 17 km. The earthquake occurred near the northern boundary of the Bayan Har block. Two highway bridges, including the Yematan bridge, collapsed. Based on the analysis of the seismic damage characteristics of the Yematan bridge, a finite element model of a three-span simply supported girder bridge was established. Nonlinear analyses were performed using the bedrock wave at the bridge site during the earthquake and the seismic safety evaluation wave of the bridge as inputs to analyze the causes of seismic damage to the bridge. The results show that the large beam displacement owing to the velocity pulse and the low-frequency components of the ground motion are the main causes of the damage to the bridge.

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Abstract. Objective: To observation and analysis one stage anterior internal fixation treatment of cervical vertebra and neck thoracic segment spine and its clinical efficacy. Methods: We select 10 patients with cervical and thoracic segments neck spine line issue of anterior cervical lesions cleared and iliac bone graft titanium plate fixation from March 2013 to August 2014. The anti-tb treatment is > 9 months after surgery. Results: surgical blood loss was 325 ml ± 25 ml. 10 cases received following-up, following-up time was 10 ~ 12 months, following-up period tuberculosis recurrence and internal fixation failure was not found. The spinal cord and nerve root compression symptoms improved to some extent, JOA score is increased from 10.8 ± 1.1 points preoperatively to 15.4 ± 0.9 points after the operation. Conclusion: One stage anterior internal fixation treatment of cervical vertebra and early cervical segmental spinal can obtain satisfactory curative effect. Thoroughly remove lesions 360 °, the treatment that jointed before and after internal fixation and bone graft system of anti-tuberculosis treatment is the key to obtain satisfactory curative effect.

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