BackgroundCentipeda minima (Ebushicao) has been used for the treatment of various diseases, such as nasal allergies, rhinitis and sinusitis, nasopharyngeal carcinoma, cough, and headache. This study aims to investigate the anticancer activities of Centipeda minima ethanol extracts (CME) against nasopharyngeal carcinoma cell CNE-1 and their underlying mechanism.MethodsCNE-1 cells were treated with different concentrations (15–50 μg/mL) of CME for different time intervals (24, 48, and 72 h). Cytotoxicity of CME was determined by MTT assay. Cell morphological changes were observed by fluorescence microscopy after HO 33258 staining. Cell cycle status was evaluated by flow cytometry following propidium iodide staining. Apoptosis was detected by flow cytometry following annexin V-FITC/PI staining. The levels of apoptosis-associated and PI3K-Akt-mTOR signaling related proteins were measured by western blotting analysis.ResultsCME (15–50 μg/mL) significantly inhibited the proliferation of CNE-1 in a dose- and time-dependent manner (P = 0.026 for 15 μg/mL, P < 0.001 for 25, 30, 40, and 50 μg/mL, respectively); the IC50 values (μg/mL) were 41.57 ± 0.17, 30.34 ± 0.06 and 24.98 ± 0.08 for 24, 48 and 72 h treatments, respectively. Significant morphological changes of CNE-1 cells displaying apoptosis were observed after CME treatment. CME showed low cytotoxicity toward normal LO2 cells. CNE-1 cells were arrested in the G2/M phase while treated with 15, 25, 40 μg/mL of CME, respectively (P = 0.032, P = 0.0053, P < 0.001). CME (15, 25, 40 μg/mL) down-regulated Bcl-2 expression (P = 0.032, P = 0.0074, P < 0.001), and up-regulated Bax (P = 0.026, P = 0.0056, P < 0.001) with activation of caspase-3, caspase-8, caspase-9, and PARP observed in CNE-1 cells (P = 0.015, P = 0.0067, P < 0.001 for caspase 3; P = 0.210, 0.028, < 0.001 for caspase 8; P = 0.152, 0.082, 0.0080 for caspase 9; P = 0.265, 0.0072, < 0.001 for PARP). CME suppressed the activation of the PI3K-AKT-mTOR pathway (P = 0.03, 0.0007, 0.004, 0.006, 0.022 for p-PI3K, p-Akt-Ser473, p-Akt-Thr308, p-mTOR-Ser2448, p-mTOR-Ser2481, respectively after 40 μg/mL of CME treated for 24 h).ConclusionCME inhibited the proliferation of CNE-1 cells and activation of the PI3K-AKT-mTOR signaling pathway.
We present a framework for example-based texture synthesis with feature directions aligned to vector fields with two way rotational symmetry, also known as orientation fields. Through a simple variational formulation, the framework allows the user to design the orientation field with intuitive controls, by interactively manipulating singularities and field directions. The resulting field is then used to guide a parallel synthesis. Our contribution is twofold: a design tool for orientation fields with a natural boundary condition, and a parallel texture synthesis adapted specifically for using such fields in feature alignment. We demonstrate the advantages of the procedure through examples on planar and curved patches with trivial topology.
Binary star systems are assumed to be co-natal and coeval, thus to have identical chemical composition. In this work we aim to test the hypothesis that there is a connection between observed element abundance patterns and the formation of planets using binary stars. Moreover, we also want to test how atomic diffusion might influence the observed abundance patterns. We conduct a strictly line-by-line differential chemical abundance analysis of 7 binary systems. Stellar atmospheric parameters and elemental abundances are obtained with extremely high precision (< 3.5%) using the high quality spectra from VLT/UVES and Keck/HIRES. We find that 4 of 7 binary systems show subtle abundance differences (0.01 - 0.03 dex) without clear correlations with the condensation temperature, including two planet-hosting pairs. The other 3 binary systems exhibit similar degree of abundance differences correlating with the condensation temperature. We do not find any clear relation between the abundance differences and the occurrence of known planets in our systems. Instead, the overall abundance offsets observed in the binary systems (4 of 7) could be due to the effects of atomic diffusion. Although giant planet formation does not necessarily imprint chemical signatures onto the host star, the differences in the observed abundance trends with condensation temperature, on the other hand, are likely associated with diverse histories of planet formation (e.g., formation location). Furthermore, we find a weak correlation between abundance differences and binary separation, which may provide a new constraint on the formation of binary systems.
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