The purpose of this study was to investigate liver and intestinal ABCA1 expression and plasma HDL-C level in response to treadmill-running training in rats. Twenty adult Wistar male rats (17-18 weeks old, 300-322 g) were divided into control (n = 10) and Training (n = 10) groups. Training group trained at 25 m/min (0% grade) for 60 min/day, 5 days/week for 12 weeks. Rats were killed 48 h after the last session of training. The intestinal and liver ABCA1 mRNA expression was found to be significantly higher in trained compared to control group (P < 0.006 and P < 0.024, respectively). Intestine and liver ATP concentrations remained unchanged. Plasma HDL-C, HDL2-C, Apo A-1, pre-beta HDL-C concentration, LCAT activity, TC/HDL-C and LDL-C/HDL-C ratio significantly increased in trained group (P < 0.01, P < 0.006, P < 0.001, P < 0.001 P < 0.067, P < 0.02, and P < 0.03, respectively). However, other lipoprotein concentrations were unchanged. In conclusion, we found that endurance training induced significant elevation in plasma HDL-C and HDL2-C concentrations, accompanied by higher plasma Apo A-1, pre-beta HDL-C concentrations, LCAT activity and ABCA1 mRNA expressions in rat intestine, and liver.
CONCLUSIONS:In a previous study, we confirmed a 40% increase in activity (iEMG) due to CAC in the rat hemi-diaphragm. Our findings indicate that satellite cell activation may be induced by extreme activation of muscle fiber without causing muscle fiber hypertrophy in young muscles. However, neither Pax7 mRNA nor satellite cells numbers was increased in the activated aged muscle. Therefore, we concluded that the potential for activation in satellite cells themselves is impaired due to aging, resulting in functional disorder of muscle regenerative ability. INTRODUCTION:The Sirt3 is a mitochondrial enzyme involved in regulation of mitochondrial energy homeostasis. Previous reports show that expression of Sirt3 is modified by exercise and diet, however, it has not been tested the effect of exercise without diet.Objective.To analyze the effect of aerobic training on Sirt3 expression in skeletal muscle of sedentary, overweight adolescents and test association with serum insulin levels. PURPOSE:The purpose of the present study was to study the effect of one session of prolong treadmill running on soleus muscle LPL mRNA and concentration and plasma LPL concentration in male Wistar rats. METHODS:All experiments in this study were conducted acoording to the policy of NIH guidelines regarding the use of animals. Twenty-four male Wisatr rats (weight: 388±31 g) randomly divided into 2 groups: control (n=12) and trained (n=12). The exercised rats ran on treadmill for 120 minutes (18 m/min, 0 º incline). Rats anesthetized, Sacrified, and blood and soleus muscle sample taken 2 and 24 h after exercise. Semi-quantitative RT-PCR was used to measure LPL mRNA level in soleus muscle. Data were analyzed using ANOVA with repeated measures. SUMMARY OF RESULTS:The results showed that LPL mRNA in soleus muscle significantly increased in 2 hours (173.14±22.34 vs. 91.71±15.38, P=0.02) and 24 hours (222.77±57.42 vs. 88.60±16.39, P=0.004) after exercise cessation in exercise vs control group. LPL concentration in soleus muscle also significantly increased in 2 hours (0.48±0.15 vs. 0.16±.16, P=0.03) and 24 hours (1.05±0.27 vs. 0.41±0.15, P=0.007) after exercise. In addition, plasma LPL concentration significantly increased 24 hours after exercise (6.6±1.43 vs. 2.32±1.51, P=0.04). CONCLUSION:The study showed that single session of prolong exercise via increasing muscle LPL gene expression can promote triglyceride hydrolysis, so ability of muscle for FFA oxidation increased and lipid metabolism improved.
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