Objective:Certain dietary constituents can induce toxicity and play a critical role in the development of several hepatic disorders. Tartrazine, metanil yellow and sunset yellow are widely used azo dyes in food products, so the present study is aimed to investigate the food color induced hepatotoxicity in Swiss albino rats.Materials and Methods:Swiss albino rats were divided into four groups, each group having six animals. Group I served as control, Group II, Group III and Group IV were administered with 25, 50 and 75 mg/kg body weight blend of sunset yellow, metanil yellow and tartrazine for 30 days. Hepatotoxicity in rats treated with a blend of these food colors was studied by assessing parameters such as serum total protein, serum albumin, serum alkaline phosphatase (ALP) as well as hepatic malondialdehyde (MDA). The activity of superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT) were assessed.Results:Significantly increased concentrations of serum total protein, serum albumin, serum ALP and hepatic MDA and significantly lowered levels of SOD, reduced GSH and CAT in the liver tissue of treated animals were observed when compared with control animals. The alteration in the liver includes necrosis of hepatocytes, infiltration and vacuolation.Conclusion:The result indicates that consumption of food color in diet induces liver tissue damage. The used doses of food color were mostly attributable to hepatocellular damage and drastic alteration in antioxidant defense system.
Objective:The present study was carried out to evaluate the toxic effect of blend of some food colors on Swiss albino rats.Materials and Methods:A blend (1:1:1) of sunset yellow, metanil yellow and tartrazine showed additive effects on serological parameters which indicate that addition of these dye together in food stuff may give rise to more toxic effects than are produced by each dye individually. Animals were divided into four groups (I, II, III, and IV). First group was treated as control and respective group of animals received 25, 50 and 75 mg/kg body weight blend of food colors by gavaging up to 30 days.Results:The serological study showed a decrease in total protein and albumin and an increase in alkaline phosphatase, SGPT and total bilirubin. The results revealed that oral administration of these blend did not affect the body weight gain.Conclusion:The prolonged consumption of the blend may cause adverse effect on human health.
Heavy metal contamination in fresh water bodies is of great concern owing to their toxicity, persistence and bioaccumulation. The current study deals with the acute toxicity of cadmium to fresh water fish, Channa punctatus. The objective of this study was to grasp the link between mortality and abnormal behavioral and morphological changes of C. punctatus exposed to cadmium chloride. Static bioassay tests were carried out to evaluate LC50 value of Cadmium (Cd) for fresh water fish, C. punctatus as well as the behavioral responses and morphological changes were also observed. Fish after treatment with various concentrations of cadmium chloride for different exposure period the percent mortality was recorded. The lowest cadmium chloride concentration at which mortality was observed as 45 mg/l. The first death of experimental fish was recorded as 125 mg/l at 24 hrs. of exposure. After 96 hrs. LC50 value of cadmium (Cd) was found to be 80.62mg/l. The major behavioral responses observed during the experiment were restlessness, jumping, erratic swimming, gulping of air at the surface, loss of equilibrium, sluggishness,opercular movements and fishes lied on the water surface before death and morphological changes like, discoloration of skin, pigmented patches on body, shedding of scales, sedimentation of chemical on body, mucous secretion, and ballooning were observed in exposed animals. The observed data showed that C.punctatus can be used as a good bio-indicator for heavy metal contamination in fresh water bodies.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.