La passion de l’ignorance, non pas comme absence de savoir mais comme quête de ce que l’on ignore, prend acte de la loi de la parole et du trou autour duquel le savoir se construit. L’ignorance consentie, avouée, permet au sujet de tendre vers une docte ignorance, soit le savoir le plus élevé, alors que l’ ignorentia docens est l’ignorance suffisante de celui qui joue au savant, qui croit savoir et passe à côté de la vérité. Dans la cure, c’est à partir d’une position d’ignorant que l’analysant s’engage dans la recherche de la vérité. Et c’est à partir de son propre non-savoir sur l’Autre que l’analyste guidera l’analysant, non pas vers un Wissen (un savoir) mais sur les voies pour y accéder, laissant le sujet venir au mot pour y reconnaître la loi de son être.
Growth, expression of functional differentiation (as characterized by synthesis and secretion of milk proteins), and primary metabolism were studied for a mouse mammary epithelial cell line, COMMA-1D, in extended-batch and hollow-fiber reactor cultures. Batch cultures were performed on Costar polycarbonate membrane inserts, allowing basal and apical exposure to medium. Protein production was induced in both batch and hollow-fiber cultures in hormone-supplemented medium. In batch cultures, high levels of protein production and secretion were maintained for 18 days. Once differentiation was induced, the rate of deinduction was low, even in medium containing epidermal growth factor (EGF) and serum; cells continued to express and secrete proteins for at least 12 days after prolactin and hydrocortisone were removed. Cells in both batch and hollow-fiber cultures were highly glycolytic and exhibited low rates of glutaminolysis. In batch culture on membrane inserts, cells showed polarized metabolism between the apical and basal side, maintaining significant gradients of glucose and lactate. Medium hormonal composition and subsequent differentiation affected both glucose uptake and lactate yield for COMMA-1D in batch culture.
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