The amino-acid sequence of a protein encodes information on its three-dimensional structure and specific functionality. De novo design has emerged as a method to manipulate the primary structure for the development of artificial proteins and peptides with desired functionality. This paper describes the de novo design of a pore-forming peptide, named SV28, that has a β-hairpin structure and assembles to form a stable nanopore in a bilayer lipid membrane. This large synthetic nanopore is an entirely artificial device for practical applications. The peptide forms multidispersely sized nanopore structures ranging from 1.7 to 6.3 nm in diameter and can detect DNAs. To form a monodispersely sized nanopore, we redesigned the SV28 by introducing a glycine-kink mutation. The resulting redesigned peptide forms a monodisperse pore with a diameter of 1.7 nm leading to detection of a single polypeptide chain. Such de novo design of a β-hairpin peptide has the potential to create artificial nanopores, which can be size adjusted to a target molecule.
Microwave
heating is widely used to accelerate the organic synthesis
reaction. However, the role of the nonthermal microwave effect in
the chemical reaction has not yet been well characterized. The microwave
heating processes of an ethanol–hexane mixed solution were
investigated using in situ microwave irradiation nuclear magnetic
resonance spectroscopy and molecular dynamics (MD) simulation. The
temperature of the solution under microwave irradiation was estimated
from the temperature dependence of the 1H chemical shifts
(chemical shift calibrated (CSC)-temperature). The CSC-temperature
increased to 58 °C for CH2 and CH3 protons,
while it increased to 42 °C for OH protons during microwave irradiation.
The CSC-temperature of CH2 and CH3 protons reflects
the bulk temperature of solution by the thermal microwave effect.
The lower CSC-temperature of the OH proton can be attributed to a
nonthermal microwave effect. MD simulation revealed that electron
dipole moments of OH groups ordered along the oscillated electric
field decreased the entropy by absorbing microwave energy and simultaneously
increased the entropy by dissipating energy to the solution as the
thermal and nonthermal microwave effect. Ordered polar molecules interact
to increase hydrogen bonds between OH groups as the nonthermal microwave
effect, which explains the lower CSC-temperature of the OH protons.
The nonthermal microwave effects contribute to the intrinsic acceleration
of the organic reaction.
Bombinin H4 is an antimicrobial peptide that was isolated from the toad Bombina variegata. Bombinin H family peptides are active against gram-positive, gram-negative bacteria, and fungi as well as the parasite Leishmania. Among them, bombinin H4 (H4), which contains d-allo-isoleucine (d-allo-Ile) as the second residue in its sequence, is the most active, and its l-isomer is bombinin H2 (H2). H4 has a significantly lower LC50 than H2 against Leishmania. However, the atomic-level mechanism of the membrane interaction and higher activity of H4 has not been clarified. In this work, we investigated the behavior of the conformations and interactions of H2 and H4 with the Leishmania membrane using P solid-state nuclear magnetic resonance (NMR), vibrational circular dichroism (VCD) spectroscopy, and molecular dynamics (MD) simulations. The generation of isotropicP NMR signals depending on the peptide concentration indicated the abilities of H2 and H4 to exert antimicrobial activity via membrane disruption. The VCD experiment and density functional theory calculation confirmed the different stability and conformations of the N-termini of H2 and H4. MD simulations revealed that the N-terminus of H4 is more stable than that of H2 in the membrane, in line with the VCD experiment data. VCD and MD analyses demonstrated that the first l-Ile and second d-allo-Ile of H4 tend to take a cis conformation. These residues function as an anchor and facilitate the easy winding of the helical conformation of H4 in the membrane. It may assist to quickly reach to the threshold concentration of H4 on the Leishmania membrane. This article is part of a Special Issue entitled: d-Amino acids: biology in the mirror, edited by Dr. Loredano Pollegioni, Dr. Jean-Pierre Mothet and Dr. Molla Gianluca.
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