The antifungal activity of Myrrh (Commiphora molmol) essential oil was tested against three storage fungi, Aspergillus flavus, A. niger and Penicillium citrinum. Results showed inhibitory effect of the oil against tested fungi with the increasing concentrations. Minimum inhibitory concentration (MIC) using agar dilution method was 4% (v/v) for A. niger and A. flavus and above 4% (v/v) for P. citrinum. Concentration (0.5-2)% (v/v) of oil also affected the sporulation of these fungi causing reduction or complete inhibition of sporulation process. Effect of Myrrh essential oil was also noticeable on spores germination, concentration of 4% (v/v) of oil inhibited complete germination of A. niger spores whereas this concentration caused 90% inhibition of A. flavus spores. Activity of vapour of Myrrh essential oil was investigated using a Petri dish atmosphere. Results showed inhibitory effect of the vapour with the increasing volume of essential oil added. MIC of vapour was (100) μl or slightly higher for all fungi.

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A 30 years married female by occupation nurse presented with left eye irritation. On Examination mild hyperemia and papillary reaction in left lower conjunctiva noted. After 2 days it converted into mild discharge and raised irritation. After taking the swab for culture and sensitivity patient discharged on antibiotics and tears substitutes. Four days later received call from pathology clinic methicillin-resistant Staphylococcus aureus (MRSA) positive from culture and sensitivity report. Emergency measures taken out for example, report the case with Infectious disease, isolation, and other sample collection and systemic to diagnosis early and towards better way of latest treatment. After 10 days of treatment patient was declare clear from MRSA. Swab, a simple test may leads us treatment. Our recommendations directed towards diagnosis, treatment and preventive measure for a nosocomial infection.