member of a class of potent cytotoxin called Shiga toxin (Stx). A subgroup of STEC causes watery diarrhoea in humans which can progress to haemorrhagic colitis and potential systemic complications due to the action of Shiga toxin (Kaper et al., 2004). These are referred as Entero haemmorhagic E.coli (EHEC). About 200 EHEC serotypes have been isolated from animal and food sources (Denis et al., 2012). The most significant EHEC of humans
Pasteurella multocida is a Gram-negative bacterium that causes haemorrhagic septicaemia in cattle and buffaloes. These organisms are involved in the formation of biofilm and can evade treatment. There is no definitive study to screen the genes associated with biofilm production associated with Pasteurella multocida. The present study describes the real-time PCR based approach for screening of genes associated with biofilm formation. Out of 10 isolates screened for biofilm formation, five of them produced biofilm on Congo red agar of which all are resistant to major antibiotics especially cotrimoxazole, nalidixic acid, enrofloxacin and tetracyclines. All the isolates show presence of genes associated with biofilm formation indicating that other factors influencing the biofilm production. We suggest that the future studies may be targeted to unravel the other factors that influence the biofilm production.
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