A nanomultilayer coating made of food-grade, bio-based materials (consisting of five nanolayers of pectin and chitosan) was produced. This coating was firstly characterized in terms of the water vapor, oxygen and carbon dioxide permeabilities; these parameters exhibited values of 0.019 ± 0.005 Â 10 À11 , 0.069 ± 0.066 Â 10 À14 and 44.8 ± 32 Â 10 À14 g m/(Pa s m 2), respectively, and are of the same order of magnitude of those found in other nanomultilayer systems. The nanomultilayer system was applied on whole 'Tommy Atkins' mangoes and the layers' adsorption was confirmed by changes in the contact angle of the coated fruits' skin. After 45 d of storage, uncoated mangoes presented a higher mass loss, higher total soluble solids and lower titratable acidity in comparison with coated mangoes. Uncoated mangoes had also a damaged and wrinkled appearance, showing evidence of microbial spoilage, and the flesh exhibited a slightly brownish color, in comparison with the coated mangoes. These results suggest a positive effect of the coating on gas flow reduction and on the consequent extension of the shelf-life of mangoes.
Nanolayered coatings of κ-carrageenan, a polysaccharide with good gas barrier properties, and lysozyme, a protein with antimicrobial action, were in a first stage assembled on aminolysed/charged polyethylene terephthalate (PET) pieces, which acted as a support, by alternate five-layer deposition. This was performed to allow the characterization of the nanomultilayer system. PET aminolysis was confirmed by Fourier transform infrared spectroscopy and contact angle, and the subsequent layer adsorption on aminolysed PET surface was confirmed by absorbance, contact angle and SEM images. The water vapour permeability and the oxygen permeability (O 2 P) of the five layers were found to be 0.013±0.003×10 −11 and 0.1±0.01×10 −14 g m −1 s −1 Pa −1 , respectively. The nanomultilayer system was subsequently applied (without PET support) directly on 'Rocha' (Pyrus communis L.) fresh-cut pears and whole pears. Uncoated fresh-cut pears and whole pears presented higher mass loss, higher total soluble solids (TSS) and lower titratable acidity when compared with coated fresh-cut pears and whole pears. Uncoated fresh-cut pears also presented a darker colour. These results showed that the nanolayered coating assembled on the fruits' surface has a positive effect on fruit quality and contributed to extend the shelf-life.
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