The regulatory region of the Streptomyces dnaA gene comprises a single promoter and two DnaA boxes that are located upstream of the promoter. Comparative analysis of the dnaA promoter region from S. chrysomallus, S. lividans and S. reticuli revealed that the location, spacing and orientation of the DnaA boxes are conserved. In vitro studies demonstrated that efficient binding of the Streptomyces DnaA protein to DNA requires the presence of two DnaA boxes. In vivo analysis of dnaA promoter mutants deleted for one or both DnaA boxes indicated that the dnaA gene is autoregulated. However, the degree of derepression observed is relatively modest.
This paper presents the findings of the inaugural season of the Bays of East Attica Regional Survey project (BEARS). The project aims to clarify the history of human activity around the bay of Porto Rafti in eastern Attica. Surface finds from Raftis Island demonstrate that it was the location of a major Late Helladic IIIC site probably linked to the cemetery at Perati, as well as of limited Late Roman occupation. The Pounta peninsula yielded a large quantity of obsidian lithics, indicating significant activity during the Final Neolithic/Early Bronze Age, with lesser quantities of material dated to the Late Helladic IIIC and Roman/Byzantine periods. At Koroni, surface finds of Late Helladic IIIC and Archaic/Classical date indicate that activity at the site predates the third-century Ptolemaic military camp excavated by American archaeologists in 1960. Overall, these survey data provide a range of new evidence and insight into the history of the Porto Rafti area and its connections to other regions of the Aegean. Methodologically, the project’s work also demonstrates the value of conducting archaeological surface survey even in areas with extensive modern development.
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