Diverse microbial communities and numerous energy-yielding activities occur in deeply buried sediments of the eastern Pacific Ocean. Distributions of metabolic activities often deviate from the standard model. Rates of activities, cell concentrations, and populations of cultured bacteria vary consistently from one subseafloor environment to another. Net rates of major activities principally rely on electron acceptors and electron donors from the photosynthetic surface world. At open-ocean sites, nitrate and oxygen are supplied to the deepest sedimentary communities through the underlying basaltic aquifer. In turn, these sedimentary communities may supply dissolved electron donors and nutrients to the underlying crustal biosphere.

The sub-seafloor biosphere is the largest prokaryotic habitat on Earth but also a habitat with the lowest metabolic rates. Modelled activity rates are very low, indicating that most prokaryotes may be inactive or have extraordinarily slow metabolism. Here we present results from two Pacific Ocean sites, margin and open ocean, both of which have deep, subsurface stimulation of prokaryotic processes associated with geochemical and/or sedimentary interfaces. At 90 m depth in the margin site, stimulation was such that prokaryote numbers were higher (about 13-fold) and activity rates higher than or similar to near-surface values. Analysis of high-molecular-mass DNA confirmed the presence of viable prokaryotes and showed changes in biodiversity with depth that were coupled to geochemistry, including a marked community change at the 90-m interface. At the open ocean site, increases in numbers of prokaryotes at depth were more restricted but also corresponded to increased activity; however, this time they were associated with repeating layers of diatom-rich sediments (about 9 Myr old). These results show that deep sedimentary prokaryotes can have high activity, have changing diversity associated with interfaces and are active over geological timescales.

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Chemical analyses of the pore waters from hundreds of deep ocean sediment cores have over decades provided evidence for ongoing processes that require biological catalysis by prokaryotes. This sub-seafloor activity of microorganisms may influence the surface Earth by changing the chemistry of the ocean and by triggering the emission of methane, with consequences for the marine carbon cycle and even the global climate. Despite the fact that only about 1% of the total marine primary production of organic carbon is available for deep-sea microorganisms, sub-seafloor sediments harbour over half of all prokaryotic cells on Earth. This estimation has been calculated from numerous microscopic cell counts in sediment cores of the Ocean Drilling Program. Because these counts cannot differentiate between dead and alive cells, the population size of living microorganisms is unknown. Here, using ribosomal RNA as a target for the technique known as catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH), we provide direct quantification of live cells as defined by the presence of ribosomes. We show that a large fraction of the sub-seafloor prokaryotes is alive, even in very old (16 million yr) and deep (> 400 m) sediments. All detectable living cells belong to the Bacteria and have turnover times of 0.25-22 yr, comparable to surface sediments.

Moore, G. F., Taira, A., Klaus, A., Becker, L., Boeckel, B., Cragg, B. A., Dean, A., Fergusson, C. L., Henry, P., Hirano, S., Hisamitsu, T. et al. (2001). New insights into deformation and fluid flow processes in the Nankai Trough accretionary prism: Results of Ocean Drilling Program Leg 190. Geochemistry, Geophysics, Geosystems, 2, Article No: 2001GC000166.The Nankai Trough accretionary prism is considered an ?end-member? prism accreting a coarse terrigenous sediment section in a setting with structural simplicity, unparalleled resolution by seismic and other geophysical techniques, and large historic earthquakes. It therefore has been the focus of Ocean Drilling Program (ODP) drilling to address several unresolved questions concerning accretionary processes and prism evolution. At six sites cored along two transects across the Nankai Trough accretionary prism during ODP Leg 190, lithostratigraphy and sediment diagenesis vary markedly. For the first time, reference sites at the seaward ends of the two transects defined the stratigraphic framework of the accreting/subducting Shikoku Basin sedimentary section. A thick section of Miocene turbidites and smectite-rich mudstone is present within the subducting section at the Ashizuri site. The turbidites and mudstones are absent in the correlative section at the Muroto site; variations in lithology, mineralogy, and hydrologic properties of the incoming sediments probably contribute to the difference in prism wedge taper between the two transects, while possibly controlling the seismic character of the active plate boundary. The d?collement in both transects is localized within a common stratigraphic unit (?5.9?7 Ma) within the lower Shikoku Basin facies. The d?collement is also a major boundary for both physical and mechanical properties. A broad low-chloride pore water anomaly in the lower Shikoku Basin unit, first identified at Site 808, progressively decreases in magnitude from prism to basin along the Muroto Transect. Physical properties relationships, evidence for mineralogic changes in the sediments, and pore fluid chemistry suggest that the chloride anomaly results primarily from in situ diagenetic reactions in the sediments, possibly augmented by flow of freshened fluid from depth. New constraints on stratigraphy and age of units along more landward parts of the Muroto Transect have dramatically changed our ideas about the tectonic evolution of the prism in this area. Growth of the seaward-most part of the prism took place very rapidly, with 40 km of accretion within the past 2 Myr. This rate is at least 3 times greater than growth rates in a comparable prism.Peer reviewe

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