Although much is known about the signals and mechanisms that lead to pathogenic interactions between plants and fungi comparatively little is known about fungal-plant mutualistic symbiotic interactions. The association between Epichloë festucae and perennial ryegrass is a powerful experimental system to understand the molecular basis for fungal-plant mutualistic interactions. We have adopted a forward genetics approach to dissect this symbiosis. Previously we described the use of plasmid mutagenesis to isolate a symbiotic mutant. We describe here the development of an Agrobacterium tumefaciens mediated T-DNA transformation system for E. festucae. Keywords: Epichloë festucae, Agrobacterium tumefaciens, TDNA, transformation
The DNA Files: Unraveling the Mysteries of Genetics ABSTR4CT The DNA Files is an award-winning radio documentary series on genetics created by SoundVision Productions. The DAMFiles was hosted by John Hockenberry and was presented in documentary and discussion format. The programs covered a range of topics from prenatal and predictive gene testing, gene therapy, and commercialization of genetic idormation to new evolutionary genetic evidence, transgenic vegetables and use of DNA in forensics. The series has been hailed as setting anew standard in science reporting on public radio. The DNA Files is distinguished by the depth and range of its coverage, due in large part to the unique partnership that SoundVision developed with its science advisors, involving them actively in the series at every stage of production. At this writing, the project has received top awards from the American Institute of Biological Sciences and the Association for Women in Communications (Clarion). The series achieved significant public radio market saturation through broadcast on an estimated minimum of 180 stations in the National Public Radio network with approximately 1 million listeners. Z?zeDNA Files is currently being broadcast, or has been broadcast, on such prestigious stations as KQED
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