The use of carrier ampholyte-free IEF (CAF-IEF) with ITP mobilization and conductivity detection in ITP mode for preconcentration and analysis of amino acids is demonstrated. The analytical procedure consists of three subsequent steps. In the first step, amino acids are continuously dosed from an infinite volume reservoir by electromigration to the column, where a sharp, stationary neutralization reaction boundary (NRB) is created in between acidic and basic primary electrolyte. Here, amino acids are selectively focused (trapped), if their pI falls to the pH difference on both sides of the NRB (pH gap). Amino acids create sharp rectangular zones, arranged according to their pI values. In the second step, focused zones are mobilized. After accumulation of the detectable amount of amino acids, dosing electrolyte in the infinite volume reservoir is changed for the mobilizing electrolyte. The migration mode is changed from CAF-IEF to ITP and substances start to migrate toward the analytical capillary. In the third step, analytes are transferred into the analytical column equipped with a conductivity detector and are detected in the new leading electrolyte in an ITP migration mode. The presented CAF-IEF-ITP-ITP with time-dependent accumulation of the large-volume sample enables to achieve in a reasonable time a 100 times lower c-LOD (here in orders of nmol/L), than can be reached by conventional hyphenated ITP-ITP.
The Žulová Upland is composed of granitoids of the Žulová batholith with relicts of Pleistocene (Elsterian) continental glaciation sediments. The investigated outcrop represents development of glacigenic sediments on rugged topography of the Žulová Upland. Investigated locality is situated on a hill located on the northern margin of the Žulová Upland. It is located near Štachlovice, local part of the Vidnava town. The exposed part of the hilltop reveals a preglacial basement covered by glacigenic sediments. The facies analysis and gravel petrology analysis of clasts with fraction 16–64 mm in b-axis were undertaken on the walls of the outcrop. The Georadar (GPR, Ground penetrating radar) was used to investigate the sedimentary landform and its relation to the basement. The granitoid basement is in places formed by elevations covered by glacigenic sediments. The height of elevation reaches 350 cmin outcrop, or ca ~400 cm according to the GPR survey. The glacitectonite, formed on the gentle side of elevation, is composed of angular blocks of granitoids of the Žulová batholith, diamicton, sand, gravel and deformed glacifluvial sand. The glacitectonite was deposited during the advancement of the continental glacier. The original subglacial cavity is enclosed by a steep side of the elevation. This cavity is filled by foreset body composed of stratified sand and gravel and nonstratified gravelly sand, gravel and diamicton. The cavity was filled by high-density turbidity currents and debris flow in subaqueous-subaerial environment. The infill of the cavity reaches ~400 cm in thickness according to the GPR survey. The cavity was filled during deglaciation in subglacial environment. The glacitectonite underlies the diamicton (supraglacial till) that was deposited as a debris flow during the retreat of the continental glacier. Unsorted gravel overlaps with erosional base the infill of the cavity, this gravel has a huge extent according to the GPR survey. This sediment represents the environment of terminoglacial stream. Gravel material of all types of glacigenic sediments is mainly composed of rocks from the Rychleby Mts. (amphibolites, Gierałtow orthogneiss, other diverse gneisses, quartzites, mica schist),quartz, and Nordic and Polish rocks. Subglacial sediments contain clasts of amphibolites (~40 %), on the other hand supraglacial and terminoglacial sediments are more polymict. Dominant subrounded shape (~60–70 %) of clasts and composition of this material indicates its origin in preglacial fluvial sediments. These fluvial sediments were deposited by river flowing from the Rychleby Mts. towards their northern foreland. The locality represents preglacial elevation of bedrock, which was glacitectonically deformed during the glaciation. Lots of different types of sediments (sub-, supra-, and terminoglacial) were deposited around the elevation during deglaciation period. The elevation was completely buried by these sediments. Deposition of these sediments was related with morphology of the elevation of bedrock. Formation of these sediments took place in environment analogous to environment of part bedrock/part till drumlin (Stokes et al. 2011).
The objective of this study were Little Ice Age (LIA) to recent subglacial tills, glaciofluvial sediments of proglacial sandur, esker sediments and sediments of morainemound complexes of Hørbyebreen polythermal valley glacier and LIA to recent sediments of lateral moraine, frontal moraine, ice-cored moraine and glaciofluvial sediments of proglacial sandur of Bertilbreen polythermal valley glacier. Fossil (probably early Holocene) subglacial and supraglacial tills and sediments of coarsegrained glaciomarginal delta of Bertilbreen have also been studied. The research focused on Old Red sandstone clasts, for which roundness, shape and striation presence have been investigated. The results from this research led to the proposal of new methodological approaches. It is mainly the covariant plot of striations and RA
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