The morphological characteristics of hybrid lines obtained from interspecific crosses between Eustoma grandiflorum and Eustoma exaltatum were analyzed based on the descriptors reported by UPOV for Eustoma grandiflorum. In the principal component analysis, a variation of 73.70% was obtained within four main components. The hybrids of the genus Eustoma were grown in weather conditions with temperature up to 44.5°C in Yucatan Mexico. The 1512063, 1512096, 1512098, and 1512100 hybrids showed a good development plant height, number of nodal segments, flower number and flower size, so that these hybrids could be cultivated tropical regions for commercial purposes.
A viable hybrid was produced by an interspecific Jatropha curcas x Jatropha integerrima cross. Artificial cross pollination was used, with J. integerrima being the pollen donor for J. curcas. Hybrid fruit resulted. Seeds from this fruit were germinated in vitro to produce seedlings as a source of leaf explants for genomic DNA extraction and purification. Molecular markers (ISSR and DAMD) were used to confirm hybridization success and characterize the combination of parental characteristics in the hybrid. Based on the band pattern, this interspecific cross resulted in genetic variation in the hybrid's DNA, since 15% of its DNA content exhibited no similarity to either parent genome. Of the remaining DNA content, 35% was similar to the female parent (J. curcas), 25% to the male parent (J. integerrima), and 25% was a combination of both parents.
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