The aim of the present study was to evaluate the clinical efficacy between manual buffy coat-derived platelet concentrates (PCs) and automated apheresis platelet concentrates (APCs) in terms of their therapeutic effects. The corrected count increment (CCI) was calculated according to detected differences in platelet concentration in patients who underwent transfusion of APCs, prepared by an automated system (group I, 72 cases) or PCs derived from buffy coat by manual method (group II, 83 cases). The clinical efficacy was assessed in terms of the CCI and clinical symptoms. The platelet contents of all the PCs were detected before transfusion. The mean 1 h CCI was 13.56±4.45 and 24 h CCI was 8.67±4.21 in group I, while the mean 1 h CCI was 15.83±4.65 and 24 h CCI was 9.57±3.36 in group II. The effective rates judged by CCI for groups I and II were 53 and 64%, respectively, and those judged by clinical symptoms were 67 and 60%, respectively. In conclusion, the clinical effectiveness of manual PCs was similar to that for APCs; thus, it could be utilized for clinical use.
OBJECTIVE To investigate the relationship of the EphA2 gene with the occurrence, invasion and metastasis of esophageal carcinoma. METHODSThe expression of EphA2 mRNA was detected by RT-PCR and the EphA2 protein was estimated by immunohistochemistry (SP method) in both esophageal cancerous tissues and normal epithelial tissues. RESULTS The expression of EphA2 mRNA showed no difference between esophageal cancerous tissues and normal epithelium, and there appeared to be no correlation with differentiation of the cancerous tissues, the depth of infiltration or lymph node metastasis (P>0.05). However, the expression of the EphA2 protein was significantly higher in cancerous tissues compared to normal epithelial tissues (P<0.05). The expression of the EphA2 protein in a deeper invasive group and in a group with lymph node metastasis was significantly higher compared to a superficially invasive group and a group without lymph node metastasis (P<0.05). Its expression did not appear to be correlated with differentiation of cancerous tissues (P>0.05). CONCLUSION The occurrence of esophagus carcinoma and the formation of invasion and metastasis may be related to overexpression of the EphA2 protein but not to the level of mRNA, a finding which may due to up-regulation at the translation leve! or by increased protein stability.T he Eph receptors are the largest known family of RTKs (receptor tyrosine kinases). The signal transduction caused by interaction of Eph receptors and their membrane-attached ligands, ephrins, play important roles in many developmental processes such as cell proliferation, differentiation and migration.f~'21 EphA2 is widely expressed in epithelial tissues including skin epithelia, intestine and lung mucosas and the ovary. Recent studies have demonstrated that EphA2 maybe involved in carcinogenesis, aggression and metastasis.I3] Eph overexpression has been observed in many human cancerous tissues such as lung, mammary, colon cancers and melanoma. [4-71 EphA2 overexpression was shown to be related to the reduction of cell-cell adhesion in invasive mammary cancer I41 and its expressional level is higher in distant metastatic focus compared to that of the primary focus in melanoma tissues.E5] Our study was performed to investigate the relationship between EphA2 overexpression and the clinicopathological factors in esophageal squamous cell carcinoma (ESCC) since it has rarely been reported.
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