Objective: The effects of lower extremity dominance on response latency and proprioceptive ability have been reported for physically active individuals, but not for sedentary individuals. The aim of this research was to explore the differences in muscle latency and proprioceptive ability between the non-dominant and dominant legs of healthy sedentary individuals. Material and Methods: Nineteen healthy male subjects without a history of any physical training practice for a minimum of 12 months were enrolled in this study. An ankle inversion tilting platform was used to measure the reaction times of the tibialis anterior and peroneus longus muscles. Joint position sense measured actively and passively and kinesthesia were used to evaluate proprioception of the ankle joint. Results: Neither the latency times of the tibialis anterior and peroneus longus muscles nor the proprioceptive ability score measurements exhibited significant differences between the non-dominant and dominant legs (p>0.05). Conclusion:The results of this investigation indicates that there is no side differences between the limbs with the evaluation of the peroneus longus or tibialis anterior muscle reaction times, ankle joint position sense, and ankle kinesthesia for healthy sedentary individuals.