The seed of Vigna angularis has long been cultivated as a food or a folk medicine in East Asia. Genistein (4′,5,7-trihydroxyisoflavone), a dietary phytoestrogen present in this plant, has been known to possess various biological properties. In this study, we investigated the possible lifespan-extending effects of genistein using Caenorhabditis elegans model system. We found that the lifespan of nematode was significantly prolonged in the presence of genistein under normal culture condition. In addition, genistein elevated the survival rate of nematode against stressful environment including heat and oxidative conditions. Further studies demonstrated that genistein-mediated increased stress tolerance of nematode could be attributed to enhanced expressions of stress resistance proteins such as superoxide dismutase (SOD-3) and heat shock protein (HSP-16.2). Moreover, we failed to find genistein-induced significant change in aging-related factors including reproduction, food intake, and growth, indicating genistein exerts longevity activity independent of affecting these factors. Genistein treatment also led to an up-regulation of locomotory ability of aged nematode, suggesting genistein affects healthspan as well as lifespan of nematode. Our results represent that genistein has beneficial effects on the lifespan of C. elegans under both of normal and stress condition via elevating expressions of stress resistance proteins.
Quercetin-3-O-α-L-rhamnopyranosyl(1→2)-O-α-L-rhamnopyranoside (QDR) was isolated from the remaining underground parts of Curcuma longa after harvesting the medicinal parts, and the antioxidant activities in vitro and lifespan-extending effect of QDR were elucidated using the Caenorhabditis elegans model system. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect of QDR showed similar potent activities in comparison with vitamin C. QDR also showed strong superoxide quenching activities as measured by the riboflavin-and xanthineoriginated superoxide quenching activities. QDR demonstrated potent lifespan extension of worms under normal culture condition. Subsequently, the protective effect of QDR on the stress conditions such as thermal and oxidative stresses was determined. In the case of heat stress, QDR-treated worms exhibited enhanced survival rate, as compared to control worms. In addition, QDR-fed worms lived longer than control worms under oxidative stress induced by paraquat. To verify the possible mechanism of QDRmediated increased lifespan and stress resistance of worms, we investigated whether QDR might alter superoxide dismutase (SOD) activity and intracellular reactive oxygen species (ROS) levels. Our results showed that QDR was able to elevate SOD activity of worms and reduce intracellular ROS accumulation in a dose-dependent manner.
Here in this study, we isolated 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG) from Curcuma longa L. and elucidated the lifespanextending effect of PGG using Caenorhabditis elegans model system. In the present study, PGG demonstrated potent lifespan extension of worms under normal culture condition. Then, we determined the protective effects of PGG on the stress conditions such as thermal and oxidative stress. In the case of heat stress, PGG-treated worms exhibited enhanced survival rate, compared to control worms. In addition, PGG-fed worms lived longer than control worms under oxidative stress induced by paraquat. To verify the possible mechanism of PGG-mediated increased lifespan and stress resistance of worms, we investigated whether PGG might alter superoxide dismutase (SOD) activities and intracellular ROS levels. Our results showed that PGG was able to elevate SOD activities of worms and reduce intracellular ROS accumulation in a dose-dependent manner.
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