This experiment was conducted to investigate the effect of pregnant mare serum gonadotrophin (PMSG) levels (300 or 600 I.U.) and the progestagen method (one sponge for 14 d or two sponge each for 7 d) on estrus response, onset and duration of estrus,lambing rate, litter size and serum progestagen concentrations during estrus period and early pregnancy. Atotal of 20 assaf ewes were used in the experiment which was conducted during April, a month that is considered as non-breading period in Palestine. Ewes were inserted with intravaginal sponges that containing 60 mg Medroxyprogesterone acetate (MAP). Seven days later, these sponge were removed and 10 new sponges were inseted to 10 of the experimental ewes. Following withdrawal of sponges, at day 14, 5ewes from each tretmant groups were injected intramauscularly with PMSG at level of 300 and 600 IU. This investigation showed that level of PMSG and progesterone application methods had no significant effects on the tested parameters, also, there are significant different in progesterone concentration between ewes gave brith to single and twines. This finding indicated that low level of PMSG can be XIII applied for estrus synchronization where lots of savings can be achieved for the benefit of local sheep farmers. Although, some slight differences were obtained but there was no significant differences between the two different progesterone application methods nor the two different PMSG doses. So one sponge followed by 300I.U. PMSG can be used to synchronize estrus successfully and with lower cost than using two sponges or high PMSG doses.
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