The tumor suppressor p53, nuclear factor-κB (NF-κB) and reactive oxygen species (ROS) have crucial roles in tumorigenesis, although the mechanisms of cross talk between these factors remain largely unknown. Here we report that miR-506 upregulation occurs in 83% of lung cancer patients (156 cases), and its expression highly correlates with ROS. Ectopic expression of miR-506 inhibits NF-κB p65 expression, induces ROS accumulation and then activates p53 to suppress lung cancer cell viability, but not in normal cells. Interestingly, p53 promotes miR-506 expression level, indicating that miR-506 mediates cross talk between p53, NF-κB p65 and ROS. Furthermore, we demonstrated that miR-506 mimics inhibited tumorigenesis in vivo, implicating that miR-506 might be a potential therapeutic molecule for selective killing of lung cancer cells.

No abstract

No abstract

A numerical model using field line tracing for modeling of three-dimensional magnetic field topology under resonant magnetic perturbations (RMPs) on experimental advanced superconducting tokamak (EAST) is presented. The topological structure is calculated in the vacuum paradigm. The modeling result predicts that the possible strike point splitting on a plasma-facing component and the lobes-like structure on the boundary are observable in the diagnostics at different locations. It is shown that the magnetic perturbations with a resonant dominant spectrum can induce a large footprint splitting effect as well as a wide stochastic layer. This is useful for observations using diagnostics with limited spatial resolution. The impact of RMP fields on marginally disconnected double null configurations is investigated. To avoid the transient heat load on the upper divertor or plasma-facing components near the upper x-point, it is necessary to keep the distance between two separatrices of a near double null configuration larger than a threshold value that depends on the RMP strength and the equilibrium properties. A preliminary RMP experiment on EAST shows that there is a good agreement between the splitting width predicted by the code and that of the particle flux measured by divertor probes. An enhancement of particle flux on the upper divertor during the RMP phase is observed in the lower single null discharge.

The naturally fractured rock in the open pit slope is susceptible to irreversible damage caused by fatigue loads related to freeze-thaw weathering, blasting vibration, earthquakes and tramcar traffic. To ensure the safety of rock mass and reveal how natural fracture affects the damage modelling characteristics is of great concern. Hence, this work aims at investigating the fatigue damage evolution of rock from volumetric deformation caused by F-T and cyclic loads. The rock structural deterioration and damage accumulation were investigated as well as the stimulated natural fracture pattern. Results show the frost heaving force acted on natural fracture results in the rock volumetric changes. The damage variable expressed by volumetric strain presents a linear relationship with freeze-thaw cycles. In addition, the axial, lateral and volumetric strain of marble exposed to cyclic loads present a two-stage pattern, they first increase quickly and then get to steady and last for a long time. A new fatigue damage model was established by considering the freeze-thaw damage and mechanical damage simultaneously. The proposed coupling damage model can well describe rock damage accumulation. Moreover, the CT images further reveal the influence of the natural fracture on rock volumetric deformation and the final damage accumulation. It is suggested that the opening-mode natural fractures contribute a lot to rock freeze-thaw deformation and fatigue deformation.

Recently, the first ever 100 s long, steady-state H-mode discharge with good control of impurities, core and edge MHD stabilities, and heat exhaust was demonstrated in the Experimental Advanced Superconducting Tokamak (EAST) using the ITER-like (International Tokamak Experimental Reactor) tungsten upper divertor. Using both radio frequency (RF) power and neutral beam injection (NBI) heating, EAST has demonstrated fully non-inductive scenarios with an extension of fusion performance at high density and low rotation: β P ∼ 2.5, β N ∼ 2.0, H98,y2 ∼ 1.2, bootstrap current fraction fBS ∼50% at q95 ∼ 6.8. With pure RF power heating, plasmas have been maintained for up to 21 s (over 40 times the current relaxation time) with zero loop voltage and small edge localized modes (ELMs) at high density (ne/nGW ∼ 0.6–0.8), β P ∼ 2.0, β N ∼ 1.6, and ƒBS ∼47%. Experimental investigations show how plasma current profiles, turbulent transport and radiation properties self-consistently evolve toward fusion relevant steady state conditions. Modeling and physics experiments have confirmed the synergistic effects between electron cyclotron heating (ECH) and low hybrid wave (LHW), where ECH enhances the heating and current drive from LHW injection, enabling fully non-inductive operation at higher density. Small/no ELMs facilitate the RF power coupling in the H-mode phase and reduce divertor erosion. A low tungsten concentration was observed at high β P with a hollow profile in the core. Reduction of the peak divertor heat flux with f rad of up to 40% was compatible with the high β P scenario by using active radiation feedback control. With features such as dominant electron heating, zero/low NBI torque and an ITER-like tungsten divertor, fully non-inductive high-performance experiments on EAST offer unique contributions towards the succesful operation of ITER and CFETR (the Chinese Fusion Engineering Testing Reactor).

No abstract

ABSTRACT. Powdery mildew and rust fungi are obligate parasites that cannot live without host organisms. They are difficult to culture in synthetic medium in the laboratory. Genomic DNA extraction is one of the basic molecular techniques used to study the genetic structure of populations. In this study, 2 different DNA extraction methods, Chelex-100 and cetyltrimethylammonium bromide (CTAB), were used to extract DNA from euonymus powdery mildew and Puccinia striiformis f. sp Tritici. Polymerase chain reaction was carried out with a race-specific-marker rDNA-internal transcribed spacer sequence. Both DNA extraction methods were compared and analyzed. The results showed that both Chelex-100 and CTAB were effective for extracting genomic DNA from infected plant tissue. However, less DNA was required for the Chelex-100 method than for the CTAB method, and the Chelex-100 method involved fewer steps, was simpler and safer, and did not require organic solvents compared to the CTAB method. DNA quality was evaluated by polymerase chain reaction, and the results showed that genomic DNA extracted using the Chelex-100 method was better than that using CTAB method, and was sufficient for Direct DNA extraction method of an obligate parasitic fungus 18547©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 18546-18551 (2015) studying the genetic structure of population.