The aim of the investigation was to study the effect of glial cell line-derived neurotrophic factor (GDNF) on animals' resistance to cerebral-ischemia-induced damage.Materials and Methods. In vivo studies were carried out on C3H male mice weighing 18-40 g. Ischemia modeling was performed by bilateral irreversible occlusion of both carotid arteries. A neurological status as well as an orientative-exploratory behavior of experimental animals and their learning capability in the post-ischemic period were analyzed by using "Open field" and "Passive avoidance" tests. In addition, high-resolution respirometer Oxygraph-2k (Oroboros, Austria) was applied to study an oxygen uptake rate of brain mitochondria in ischemic conditions.Results. GDNF application in bilateral occlusion of carotid arteries was found to contribute to the neurological status recovery. Moreover, it normalizes oxygen uptake rate of mitochondria in the post-ischemic period.Conclusion. GDNF has a marked neuroprotective and antihypoxic effect under ischemia modeling in vivo.Key words: glial cell line-derived neurotrophic factor; GDNF; neuroprotection; cerebral ischemia. During several years the number of ischemic brain injury incidents has been dramatically increased, that determines topical and socially important issue for modern neurology and neuroscience. The consequences of cerebral ischemia are directly related to memory and neurological status deteriorations as well as to impairment of learning capabilities and cognitive functions. Therefore, the development of modern techniques to effectively protect the nervous system from those damaging effects is urgently needed. A promising approach to improve adaptive capabilities of nervous system supposed to the activation of endogenous systems promoting the survival of nervous cells under stress factors and maintenance their functional activity. The use of neurotrophic factors such as glial cell linederived neurotrophic factor (GDNF) is of special interest. These proteins are attracted of scientists' attention because of their possibilities to regulate neurogenesis and the functioning of the nervous cells not only in early ontogenesis, but also in an adult brain. Moreover, they are involved in the processes of synapses formation, and have a pronounced effect on growth Biomedical investigations
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