Several thermophilic Bacillus strains were isolated from natural as well as artificial habitats. They grow optimally on a carbohydrate-containing medium at a temperature of 65 to 68 "C and a pH value of 6 to 7 under aerobic conditions. They utilize glucose, sucrose and sodium acetate as carbon and energy sources. They can be differentiated by acid formation and composition of intracellular fatty acid fraction as well as growth on xylose, lactose, starch, cellobiose, ribose and galactose.
A thermostable α‐amylase catalyzed the exothermal hydrolysis of cyclodextrins. It was immobilized covalently via a spacer on controlled pore glass (CPG‐10) or Silicagel. The temperature signal caused by the reaction heat of the cyclodextrin hydrolysis was determined in a one column calorimetric system (enzyme thermistor). It was correlated to the cyclodextrin concentration and depended on the type of enzyme carrier and kind of cyclodextrin hydrolyzed. The proposed technique offers a direct route to the determination of α‐amylase activity, and the results are of importance for analysis of cyclodextrin concentration.
A thermophilic. bacterial mixed culture was isolated from spent liquor of pulp mills, using a chemostat under aerobic conditions. It consists of two components belonging to genus Bacillus. The thermophilic Bacilli were cultivated aerobically and continuously at 67-70 "C and pH 6.8-7, rising the percentage of spent liquor in stages. The dilution rate ranged from 0.2 to 0.33 h-' during the experiments. The composition of the cell mass produced was analyzed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.