Pharmacokinetic interaction between ciprofloxacin, an antibacterial fluoroquinolone and flavonoid quercetin was investigated in the present study. Male wistar albino rats, weighing about 200-250 g were randomly divided into two groups, six rats in each group. Rats in group I (control) received ciprofloxacin alone (10 mg/kg PO). Group II received ciprofloxacin 30 min after pretreatment with quercetin (20 mg/kg PO). Rats were anaesthetized after ciprofloxacin administration for blood collection with a xylazine-ketamine combination. Blood samples were collected from jugular vein and tail clipping at predetermined time intervals prior to and at 0.33, 0.67,1, 1.5, 2, 4, 6, 8 and 12 h (20 , 40 , 6 0, 90, 12 0, 240 , 3 60, 48 0 a nd 720 min.) time interva ls after administration of ciprofloxacin. Peak plasma concentrations of ciprofloxacin will be reached in 1-2 h. after oral administration in rats. Plasma was separated by centrifuging at 3000 rpm for 10 min and stored at -20 o C until analyzed for ciprofloxacin by microbiological assay, using Escherichia coli (ATCC 25922). Based on plasma concentrations, the pharmacokinetic parameters were determined by non compa rtmenta l methods. Detectable plasma concentrations of ciprofloxacin in ra ts persisted upto 6 h when ciprofloxacin was given alone whereas ciprofloxacin was detectable up to 8 h in quercetin pretreated rats. Prior administration of quercetin modified the kinetic profile of ciprofloxacin as evidenced by the significantly (p<0.01) higher area under curve (AUC), area under the first moment curve (AUMC) and there was significant (p<0.01) decrease in V dss and Cl B when compared to untreated group.
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