Microsatellites, also known as simple sequence repeats (SSRs), are the class of repetitive DNA sequences present throughout the genome of many plant and animal species. Recent advances in molecular genetics had been the introduction of microsatellite markers to investigate the genetic structuring of natural plant populations. We have employed an enrichment strategy for microsatellite isolation by using multi-enzymes digestion, microsatellite oligoprobes, and streptavidin magnetic beads in Sesamum (Sesamum indicum L.). More than 200 SSR motifs were detected (SSR motifs ≥2 repeat units or 6 bp); 80 % of the clones contained SSR motifs. When regarding SSRs with four or more repeat units and a minimum length of 10 bp, 132 of them showed repeats. Eighteen SSR markers were initially characterized for optimum annealing temperature using a gradient PCR technique. Among the 18 SSR markers characterized, five were found to be polymorphic and used to analyze 60 Sesamum germplasm accessions. The maximum number of alleles detected was four with a single primer and the least number of two alleles with three primers with an average PIC value of 0.77. SSRs are a valuable tool for estimating genetic diversity and analyzing the evolutionary and historical development of cultivars at the genomic level in sesame breeding programs.
Science, as a subject is universal and knows no boundaries. The claims of Science for inclusion in the school curriculum came to be recognized after years of active and persistent efforts. Many students find science extremely inspiring and interesting, however when amply motivated. Pre-test and post-test experimental research design, was adopted for the study. A sample of 219, 8th class students (169 female, 50 male students) from six Ashram high schools of Warangal district was selected. Students' Motivation Towards Science Learning (SMTSL) (Tuan et al. [1]) scale was adopted in this study. The sum of SMTSL hiked to 87.38% from 73.28% in post-test. This increase was attributed to the intervention through tutoring students with video lessons and evaluating with quizzes before and after screening video lessons. The variable Performance goal, contributed to the maximum (33.62%). A shift in students from moderately motivated category to highly motivated category was also found in post intervention situation.
Thioesterases play a central role in determining chain lengths of fatty acids in oil storage tissues and have been isolated from a number of plant sources. TEs can be classified into two evolutionarily related families, namely FatA and FatB, with distinct substrate selectivity. With the help of bioinformatics tools alignment was done among the selected thioesterase genes from related oilseed crops. Degenerate primers were designed by manual, codehop and webprimer methods for the conserved regions. Gradient PCR with designed primers showed amplification with manual and codehop primers in sesamum DNA. Amplified DNA is transformed and plasmids are sequenced. Sequences are searched for homology with BLAST. The homology at the amino acid level for thioesterase sequences was observed to be with Helianthus annuus than with the other oil seed crops compared.
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