After absorption with selected individual rabbit sera, allo‐antibodies directed against the a‐negative IgG from homozygous a2‐suppressed rabbit resulted in the separation of three distinct antibody populations which are designated anti‐A31, anti‐x32 and anti‐y33. The mating studies indicated that the x32 and y33 allotypic specificities are not controlled by allelic alternatives. The inheritance of the x32 and y33 allotypes was found to be closely linked to that of the al, a2 or a3 allotypes. Five allogroups were identified: a1, x−,y−;a1,x−,y33;a1,x32,y33;a2x32,y33; and a3,x32,y−. As was found for the a1, a2 and a3 allotypic specificities, the x32 and y33 allotypic specificities are present on IgA and IgM as well as on IgG suggesting that the x32 and y33 allotypic specificities are located on the variable region of immunoglobulin heavy chains. Our results indicate that three closely linked VH genes code for the variable regions of rabbit Ig heavy chain subgroups.
The objective of this study was to investigate single nucleotide polymorphisms (SNPs) in the bovine nephroblastoma overexpressed (NOV) gene and to evaluate whether these polymorphisms affect carcass traits in the Korean cattle population. We resequenced to detect SNPs from 24 unrelated individuals and identified 19 SNPs within the full 8.4-kb gene, including the 1.5-kb promoter region. Of these 19 SNPs, four were selected for genotyping based on linkage disequilibrium (LD). We genotyped 429 steers to assess the associations of these four SNPs with carcass traits. Statistical analysis revealed that g.7801T>C and g.8379A>C polymorphisms in the NOV gene were associated with carcass weight (p = 0.012 and 0.008, respectively), and the g.2005A>G polymorphism was associated with the back fat thickness (BF) trait (p = 0.0001). One haplotype of the four SNPs (GGTA) was significantly associated with BF (p = 0.0005). Our findings suggest that polymorphisms in the NOV gene may be among the important genetic factors affecting carcass yield in beef cattle.
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