Recent works suggest that the combination of several PGPRs could be more effective than individual strains as a horticultural product. LS213 is a product formed by a combination of two PGPRs, Bacillus subtilis strain GB03 (a growth-promoting agent), B. amyloliquefaciens strain IN937a (an inducer of systemic resistance) and chitosan. The aim of this work is to establish if the combination of three PGPR, B. licheniformis CECT 5106, Pseudomonas fluorescens CECT 5398 and Chryseobacterium balustinum CECT 5399 with LS213 would have a synergistic effect on growth promotion and biocontrol on tomato and pepper against Fusarium wilt and Rhizoctonia damping off. When individual rhizobacterium and the LS213 were put together, the biometric parameters were higher than with individual rhizobacterium both in tomato and pepper, revealing a synergistic effect on growth promotion, being the most effective combination that of B. licheniformis and LS213. When P. fluorescens CECT 5398 was applied alone, it gave good results, which could be due to the production of siderophores by this strain. Biocontrol results also indicate that those treatments that combined LS213 and each of the bacteria (Treatments: T7 and T8) gave significantly higher percentages of healthy plants for both tomato (T7: 65%) and pepper (T7: 75% and T8: 70%) than the LS213 alone (45% of healthy plants for tomato and 60% for pepper) three weeks after pathogen attack. The effects in pepper were more marked than in tomato. The best treatment in biocontrol was the combination of P. fluorescens and LS213. In summary, the combination of microorganisms gives better results probably due to the different mechanisms used.
In recent years, soil inoculation with cyanobacteria has become one of the most promising biotechnological strategies for restoring soil functionality in degraded drylands because of their critical role in increasing soil fertility and preventing erosion.Nevertheless, in order to fully exploit this biotechnology on a large scale, it must still be shown whether inoculated cyanobacteria are capable of developing in soils with different physicochemical properties, and new candidates adapted to desert conditions must be explored. To evaluate the potential of cyanobacteria for restoring soil functions of degraded dryland soils, in this laboratory study, we analyzed the effect of inoculating three native N-fixing species (Nostoc commune, Scytonema hyalinum, and Tolypothrix distorta), individually and as a consortium, on soil properties from three different semiarid ecosystems in southeast Spain. The biocrust colonization was monitored by determining chlorophyll a content (the typical surrogate used for biocrust biomass). Other methodologies, such as the analysis of soil spectral response and image classification were also applied for cover estimation of the biocrust. After 3 months, all inoculated soils showed cyanobacteria cover of up to 50%, lower albedo and higher chlorophyll a content. Cyanobacterial inoculation also improved soil functions, as they promoted a significant gain in total organic carbon and total nitrogen in all soils. Among inoculation treatments, Nostoc commune and the mixture of all three species promoted the most cyanobacteria coverage, chlorophyll content, and surface darkening, as well as organic carbon and total nitrogen gains in the soil, highlighting their excellent performance in biocrust development.
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