Conjugated linoleic acid (CLA) is a collective term for positional and geometric isomers of linoleic acid. Dietary CLA has been shown to improve feed efficiency, decrease body fat, and increase lean tissue in laboratory animals. We hypothesized that CLA would improve performance and carcass composition and would be deposited in pork tissues. Diets of 40 crossbred pigs were supplemented with CLA to determine its effects on performance and carcass composition. Eight replications of five littermate barrows with an initial average weight of 26.3 kg were allotted at random to individual pens. Within replication dietary treatments containing 0, 0.12, 0.25, 0.5, or 1.0% CLA were assigned at random. Pigs were weighed and feed disappearance was determined at 14-d intervals. Average daily gain increased linearly as the level of CLA increased in the diet (P < 0.05). Average daily feed intake was not affected by the concentration of CLA in the diet. Therefore, a linear increase in gain:feed ratio (P < 0.05) was observed. Carcasses from animals fed control diets had greater 10th rib backfat than carcasses from animals fed CLA (P < 0.05). Ultrasound measurement and carcass measurements showed less fat depth over the loin eye at the 10th rib of pigs fed doses of CLA (P < 0.05) than that observed for control pigs. Belly hardness (firmness) increased linearly as the concentration of CLA in the diet increased when bellies were measured for firmness either lean side up (P < 0.001) or lean side down (P < 0.05). Loin dissection data demonstrated that CLA produced a quadratic treatment effect both for less intermuscular fat (P < 0.001) and less subcutaneous fat (P < 0.05) and a linear increase for bone (P < 0.05), although finished loin weight only tended to increase (P = 0.08). The CLA concentration increased in a linear relationship in both subcutaneous fat (P < 0.001) and lean tissue (P < 0.001). Dietary CLA was incorporated into pig tissues and had positive effects on performance and body composition.

Conjugated linoleic acid (CLA) was fed to growing-finishing barrows (n = 92) at 0.75% of the diet. A commercial CLA preparation (CLA 60) containing 60% CLA isomers was included at 1.25% to provide 0.75% CLA in the diet. The inclusion of CLA in diets was initiated at various BW and fed until slaughter. Growth, carcass, meat quality, physical, chemical, and sensory data were collected and analyzed. Treatments T1, T2, T3, and T4 included the last 0, 29, 56 and 87 kg, respectively, of weight gain before slaughter. Average daily gain and feed intake were not affected (P > or = 0.06) by CLA, but gain:feed responded quadratically (P = 0.05), over the entire BW gain (28 to 115 kg) with pigs of T2 and T3 having the greatest gain:feed. Loin muscle area increased (P = 0.01) linearly with increasing weight gain while fed CLA, and 10th rib, first rib, and last rib fat depth decreased (P < or = 0.05) linearly. Subjective quality measures on loin muscles increased linearly for marbling (P < 0.05) and tended to increase for firmness (P = 0.07) with increasing weight gain while barrows were fed CLA. Objective Hunter color values for loin chops from T1 and T4 were not different for L* (P = 0.12) or a* (P = 0.08) values but were higher (P < 0.05) for b* values with CLA feeding. Lipid oxidation values of loin muscle tissue were lower (P < 0.05) for pigs fed CLA (T1 vs T4). Increasing the period of weight gain while feeding CLA linearly increased (P < 0.01) saturated fatty acids and CLA isomers in loin tissue and linearly increased (P < 0.01) saturated fatty acids and CLA isomers in subcutaneous adipose tissue. Sensory panel characteristics of loin chops were not changed (P > 0.05) by feeding CLA. Increased gain:feed, increased loin muscle area, decreased fat depth, and improvements in marbling and firmness with CLA feeding could result in improved profitability of pork production systems.

Conjugated linoleic acid (CLA) was supplemented to crossbred growing-finishing barrows (n = 60) at 0.75% of the total diet. Pigs were randomly assigned to the CLA or control diets based on stress genotype (negative, carrier, or positive). Gain:feed was higher for CLA diet animals (350 g/kg feed) than for control diet animals (330 g/kg feed) independent of genotype (P < 0.05). No differences were observed for ADG for the diets (P = 0.71) or genotype classes (P = 0.40). Postmortem pH was lower (P < 0.01) by 3 h for CLA-supplemented pigs, with no differences in ultimate pH. No differences (P = 0.16) were observed for ultimate pH between the three genotypes. Conjugated linoleic acid-supplemented pigs exhibited less 10th rib fat depth (2.34 cm vs 2.84 cm) and last rib fat depth (2.46 cm vs 2.72 cm) than control pigs (P < 0.05). Loin muscle area (LMA) was not affected (P = 0.18) by CLA supplementation, but LMA was different (P < 0.02) for genotype; positive genotype carcasses had the largest LMA (45.02 cm2) and negative carcasses had the smallest LMA (36.44 cm2). Carrier carcasses were intermediate for LMA (40.76 cm2). Subjective scores for color were not affected (P = 0.98) by CLA but color was different (P < 0.01), with scores of 1.50, 2.40, and 3.1 for positive, carrier, and negative genotypes, respectively. Subjective marbling scores were increased (P < 0.03) in all genotypes with CLA supplementation. Subjective firmness scores were higher (P < 0.06) for CLA-supplemented pigs and were highly correlated (0.89) to marbling scores. The L* values were higher (P < 0.01) for stress-positive pigs at 24 h postmortem. Also, L* values were higher (P < 0.01) for CLA-fed pigs over 7 d of shelf storage. Sensory characteristics were not different with CLA supplementation for tenderness (P = 0.24), juiciness (P = 0.35), or flavor intensity (P = 0.14). This study showed that LMA was increased with stress-carrier and stress-positive genotypes, but lean color was negatively affected with the presence of the stress gene. Conjugated linoleic acid supplementation improves feed efficiency, decreases backfat, and improves pork quality attributes of marbling and firmness of the longissimus muscle. Furthermore, there is seemingly no interaction between the stress-genotype status of pigs and the subsequent effect of CLA on their growth and performance.

An evaluation of porcine longissimus myoglobin concentration was conducted to determine breed and gender differences for myoglobin content, estimate genetic parameters for myoglobin concentration, and determine the relationship between myoglobin content and objective measures of muscle color. Data from centrally tested (n = 255), purebred Yorkshire (42), Duroc (61), Hampshire (17), Chester White (28), Berkshire (67), Poland China (28), and Landrace (12) barrows and gilts from the 1999 National Barrow Show Sire Progeny Test were used. Ultimate pH and Hunter L were measured on the 10th-rib face 24 h postmortem. A section of bone-in loin containing the 10th rib was taken to the Iowa State University Meats Laboratory. At 48 h postmortem, Hunter L, CIE L*, a*, and b*, Japanese color score, and water-holding capacity were measured on the face of the 10th-rib loin chop. A slice from the 10th-rib loin section was evaluated for percentage of i.m. fat. The resulting loin chop was used for the determination of soluble myoglobin concentration (mg/g, wet basis). Chester White, Hampshire, and Duroc pigs had the highest (P < 0.05) myoglobin concentration (0.92, 0.95, and 0.85 mg/g, respectively), whereas Landrace had the lowest (0.62 mg/g; P < 0.05). No gender differences were detected for myoglobin concentration. The heritability estimate for soluble myoglobin concentration was 0.27. Residual correlations between soluble myoglobin and CIE L*, a*, b*, Hunter L (24 h), Hunter L (48 h), and Japanese color score were -0.17, 0.23, -0.15, -0.16, -0.13, and 0.13, respectively. These correlations are low but in the desired direction. The residual correlation between soluble myoglobin and intramuscular fat percent was 0.18. Results show that myoglobin concentration has a moderate heritability and could be used in a selection program to make pork loins darker in color.

A total of 54 finishing barrows (initial BW = 99.8 ± 5.1 kg; PIC C22 × 337) reared in individual pens were allotted to 1 of 6 dietary treatments in a 2 × 3 factorial arrangement of treatments with 2 levels of ractopamine (0 and 7.4 mg/kg) and 3 levels of dietary energy (high, 3,537; medium, 3,369; and low, 3,317 kcal of ME/kg) to determine the effects of dietary ractopamine and various energy levels on growth performance, carcass characteristics, and meat quality of finishing pigs. High-energy diets were corn-soybean-meal-based with 4% added fat; medium-energy diets were corn-soybean meal based with 0.5% added fat; and low-energy diets were corn-soybean meal based with 0.5% added fat and 15% wheat middlings. Diets within each ractopamine level were formulated to contain the same standardized ileal digestible Lys:ME (0 mg/kg, 1.82; and 7.4 mg/kg, 2.65 g/Mcal of ME). Individual pig BW and feed disappearance were recorded at the beginning and conclusion (d 21) of the study. On d 21, pigs were slaughtered for determination of carcass characteristics and meat quality. No ractopamine × energy level interactions (P > 0.10) were observed for any response criteria. Final BW (125.2 vs. 121.1 kg), ADG (1.2 vs. 1.0 kg/d), and G:F (0.31 vs. 0.40) were improved (P < 0.001) with feeding of ractopamine diets. Feeding of the low-energy diet reduced (P = 0.001) final BW and ADG compared with the high- and medium-energy diets. Gain:feed was reduced (P = 0.005) when the medium-energy diets were fed compared with the high-energy diets. Additionally, G:F was reduced (P = 0.002) when the low-energy diets were compared with the high- and medium-energy diets. Feeding ractopamine diets increased (P < 0.05) HCW (93.6 vs. 89.9 kg) and LM area (51.2 vs. 44.2 cm(2)). The LM pH decline was reduced (P ≤ 0.05) by feeding ractopamine diets. The feeding of low-energy diets reduced (P = 0.001) HCW when compared with the high- and medium-energy diets and reduced (P = 0.024) 10th-rib backfat when compared with the high- and medium-energy diet. These data indicate that feeding ractopamine diets improved growth performance and carcass characteristics, while having little or no detrimental effect on meat quality. Reductions in energy content of the diet by adding 15% wheat middlings resulted in impaired ADG, G:F, and 10th-rib backfat. There were no ractopamine × energy level interactions in this trial, which indicates that the improvements resulting from feeding ractopamine were present regardless of the dietary energy levels.