Aim:The objective of this study was to investigate the chromosomal profile of indigenous pigs by computing morphometric measurements.Materials and Methods:A cytogenetic study was carried out in 60 indigenous pigs to analyze the chromosomal profile by employing the short term peripheral blood lymphocyte culture technique.Results:The modal chromosome number (2n) in indigenous pigs was found to be 38 and a fundamental number of 64 as in the exotic. First chromosome was the longest pair, and thirteenth pair was the second largest while Y-chromosome was the smallest in the karyotype of the pig. The mean relative length, arm ratio, centromeric indices and morphological indices of chromosomes varied from 1.99±0.01 to 11.23±0.09, 1.04±0.05 to 2.95±0.02, 0.51±0.14 to 0.75±0.09 and 2.08±0.07 to 8.08±0.15%, respectively in indigenous pigs. Sex had no significant effect (p>0.05) on all the morphometric measurements studied.Conclusion:The present study revealed that among autosomes first five pairs were sub metacentric, next two pairs were sub telocentric (6-7), subsequent five pairs were metacentric (8-12) and remaining six pairs were telocentric (13-18), while both allosomes were metacentric. The chromosomal number, morphology and various morphometric measurements of the chromosomes of the indigenous pigs were almost similar to those established breeds reported in the literature.
Karyological investigation pertaining to 30 crossbred pigs (both sexes) maintained at AICRP, Tirupati was undertaken in order to study the chromosomal profile of the crossbred pigs and their morphometric measurements. The cells had the usual diploid complement of 38 chromosomes (2n = 38) and a fundamental number of 64 as in the exotic pigs with XY complement in males and XX complement in females. The karyotype of crossbred pig was characterized by 5 pairs of submetacentric chromosomes (1-5), next two pairs were sub telocentric (6-7), subsequent 5 pairs were metacentric (8-12) and remaining six pairs were telocentric (13-18). A detailed idiogram was constructed from the results obtained. First chromosome was the longest pair and thirteenth pair was the second largest, while Ychromosome was the smallest in the karyotype of the pig. The X chromosome was metacentric, but not readily distinguishable from the autosome pair number 9, while Y chromosome was metacentric.
Highlights
First study in sheep that compared the efficacy of different commercially available media on in vitro culture of individually isolated preantral follicles.
First study in sheep that investigated the influence of VEGF, Estradiol-17
β
, GDF-9 and FGF on the growth of individually isolated preantral follicles.
VEGF, Estradiol-17
β
, GDF-9 and FGF all have beneficial influence on the development of sheep PFs’ cultured in TCM 199B.
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