Exploration for promising polyhydroxyalkanoates (PHA) producers is a continuous process as effort to mitigate the accumulation of synthetic plastic is on the rise. The present study focuses on isolation of indigenous polyhydroxyalkanoates producing bacteria from soil, waste water effluent and organic waste sample of Obio-Akpor, LGA. Screening of the selected bacteria isolates for polyhydroxyalkanoates production was based on viable plate method followed by microscopic visualization using Sudan black B. The PHA producing abilities of the selected isolates were estimated by gravimetric method and the positive isolates were detected for the presence of PHA synthase gene and identified up to genus level. Results revealed that out of 106 isolates, 55 were suspected to accumulate PHA after screening and produced PHA in the range of 0.2-1.1 g/L. Six potential isolates produced highest PHA yield and their phaC genes were successfully amplified from genomic DNA. Based on morphological, cultural and biochemical characteristics, the six isolates were tentatively identified to the genus Bacillus sp, Lysinibacillus sp and Pseudomonas sp. The result of the present study indicated that these bacterial isolates have the potential of producing PHA and their potential harnessed for future purposes.
Polyhydroxyalkanoates (PHA) are renewable, biodegradable biopolymer intracellularly accumulated by wide array of microorganisms as carbon reserve. This study investigates the influence of various cultural conditions on PHA production by a recently isolated local species under submerged fermentation. Six PHA producing strains were identified by 16S rDNA gene sequencing and strain Priestia flexa OWO1 showed satisfactory PHA productivity. The effects of production parameters were investigated and extraction of PHA was carried out using sodium hypochlorite method and maximum amount was detected after 72h. Maximum PHA production was obtained at incubation period of 48h, pH of 7.0 and temperature of 30oC. Amongst the hydrolysate of agro waste used, brewers spent grain (BSG) gave maximum yield of 3.01g/L while beans bran powder gave the highest PHA yields of 3.9 g/L amongst the organic nitrogen sources tested. Analysis of the crude PHA by Fourier Transform Infrared Spectroscopy (FT-IR) showed the presence of methyl, methylene as well as carbonyl functional groups. PHA production was higher after optimizing the production conditions as compared to basal medium therefore the utilization of these cheap renewable resources as alternative substrates for production of PHA make the process cost effective and sustainable.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.