The composition and content of individual phenolic compounds in various fruit wines and overall antioxidant properties of these fruit wines were studied. Phenolic compounds were separated by reverse phase (RP) HPLC techniques, and their content was determined by means of mass spectrometer (MS) with triple quadrupole (TQ) analyser, which recorded specific precursor-product transitions. Antioxidant activity was monitored by the following spectrophotometric methods: DPPH, FRAP and Folin-Ciocalteu assay, respectively. Four types of berries (raspberry, blackberry, chokeberry and blueberry), one type of pome (apple) and one type of stone fruit (cherries) were used for the production of fruit wines. Corresponding fruit wines were produced by microvinification with or without adding sugar into the must before fermentation. Increase in alcohol level was responsible for the increased content of phenolic compounds in fruit wine due to improved extraction conditions. Produced fruit wines were preserved by adding SO 2 which interferes with the determination of antioxidant activity of phenolic compounds. In this case, the development of a correlation method based on FRAP assay was introduced. Fruit wines are a rich source of substances which show beneficial effects on human health. Depending on the fruit type, different antioxidant compounds were predominant in wine samples.

An ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS) method with multiple reactions monitoring mode (MRM) has been developed and validated for quantification of vanillin and syringaldehyde in plum brandy. The method showed good linearity (0.05 to 10 mgL?1) and low limits of detection and quantification (LOD and LOQ were 11.6 ?gL?1 and 38.2 ?gL?1 for vanillin, and 12.7 ?gL?1 and 42.0 ?gL?1 for syringaldehyde, respectively). The overall intra-day and inter-day variations were less than 4.21%, and the overall recovery over 93.0%. The correlation coefficients (R2) of the calibration curves were higher than 0.9999. In order to evaluate if the method is suitable for use as a routine analytical tool, in 31 Serbian plum brandy samples vanillin and syringaldehide were determined. [Projekat Ministarstva nauke Republike Srbije, br. 172053]