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This paper presents the approximate analytical expression for transient and steady-state concentration profiles of enzymes, mediator, substrate and current. The transport and kinetics of the reaction in the diffusion layer with a rotating disc electrode are described using closed-form solutions of homogeneous systems. These new approximate analytical expressions are valid for all values of parameters. Furthermore, in this work, the numerical simulation is also presented using the Matlab program. The analytical results are compared with simulation results, and satisfactory agreement is noted.

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