and, more recently, enzyme-linked immunosorbent assay (ELISA). NATURE OF C. IMMITIS C. immitis exists in nature and in the usual culture media in its saprobic form: septate hyphae 2 to 4 Rm in diameter which, in 5 to 7 days, yield a chain of multinucleate arthroconidia which usually alternate with smaller, nonviable, brittle cells (Fig. 1). The latter degenerate, thus releasing the arthroconidia, which readily become airborne. The arthroconidia can germinate to yield new hyphae or can serve as the form infecting humans and other hosts. When inhaled, the arthroconidia, in the presence of the phagocytic cells (42) and increased CO2 (80), convert into a different morphologic form. Shedding an outer wall layer and all but one nucleus, the arthroconidia round up and enlarge to produce an immature spherule. The nucleus undergoes division, which is followed by partitioning of the cytoplasm by inward extension of the cell wall. Completion of the nuclear division and segmentation produce a mature spherule with endospores within 48 h in vivo and at 30 to 96 h in vitro. Following the segmentation, the endospores become